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Contributor Information

  • Name SimĂŁo Teixeira da Rocha
  • Institute Instituto de Medicina Molecular JoĂŁo Lobo Antunes

Tool Details

  • Tool name: Xist-TetOP mutant: ?B+1/2C cell line
  • Tool type: Cell Lines
  • Tool sub-type: Continuous
  • Parental cell line: Xist-TetOP XY ESCs (Wutz et al., 2002 PMID: 11780141)
  • Organism: Mouse
  • Description: Deletion of mouse Xist lncRNA to dissect specific RNA modules in the process of X-chromosome inactivation.
  • Research area: Epigenetics & Nuclear Signalling ; Stem Cell Biology

  • For Research Use Only

Target Details

Application Details

Handling

  • Format: Frozen
  • Growth medium: ESCs grow in feeder-free classic ESC medium DMEM media containing 15% fetal bovine serum (FBS), 103 U/ml leukemia inhibitor factor (LIF), 10-4 mM 2-mercaptoethanol, 50 U/ml penicillin, and 50 ?g/ml of streptomycin (Gibco) at 37°C in 5-8% CO2. Medium is changed daily Inducible expression of Xist driven by a TetO promoter is achieved by adding DOX (1.5 ?g/ml).
  • Shipping conditions: Dry ice

Documentation

  • Available on request

References

  •   Bousard et al. 2019. EMBO Rep. 20(10):e48019. PMID: 31456285.