Product Image

Contributor Information

  • Name Sim?„ƒo Teixeira da Rocha
  • Institute Instituto de Medicina Molecular Jo?„ƒo Lobo Antunes

Tool Details

  • Tool name: Xist-TetOP mutant: DeltaB cell line
  • Tool type: Cell Lines
  • Tool sub-type: Continuous
  • Parental cell line: Xist-TetOP XY ESCs (Wutz et al., 2002 PMID: 11780141)
  • Organism: Mouse
  • Growth properties: Partial disruption of Polycomb recruitment to the inactive X-chromosome.
  • Model: Stem Cells
  • Conditional: No
  • Conditional description: Inducible through addition of doxycycline (1.5 ÎĚ?Ÿg/ml) to the medium
  • Description: Deletion of mouse Xist lncRNA to dissect specific RNA modules in the process of X-chromosome inactivation.
  • Research area: Genetics; Stem cell biology
  • Production details: CRISPR/Cas9 genome editing of the Xist-TetOP XY ESC line
  • Additional notes: Cancer Research Technology Limited (trading research tools as Ximbio) has been granted a non-exclusive license to the CRISPR-Cas9 technology by ERS Genomics Ltd under the patent rights listed here. This license from ERS Genomics Ltd allows Ximbio to develop and commercialise CRISPR-Cas9 modified cell lines for research use only. Ximbio can provide these modified CRISPR-Cas9 cell lines to companies under a label-use only license.

  • For Research Use Only

Target Details

  • Target: Mouse Xist lncRNA

Application Details


  • Format: Frozen
  • Growth medium: ESCs grow in feeder-free classic ESC medium - DMEM media containing 15% fetal bovine serum (FBS), 103 U/ml leukemia inhibitor factor (LIF), 10-4 mM 2-mercaptoethanol, 50 U/ml penicillin, and 50 ?g/ml of streptomycin (Gibco) at 37?‚°C in 5-8% CO2. Medium is changed daily Inducible expression of Xist driven by a TetO promoter is achieved by adding DOX (1.5 ?g/ml).
  • Shipping conditions: Dry ice



  •   Bousard et al. 2019. EMBO Rep. 20(10):e48019. PMID: 31456285.