CELL LINES
Contributor Information
- Name SimĂÂo Teixeira da Rocha
- Institute Instituto de Medicina Molecular JoĂÂo Lobo Antunes
Tool Details
- Tool name: Xist-TetOP mutant: ?C cell line
- Tool type: Cell Lines
- Tool sub-type: Continuous
- Parental cell line: Xist-TetOP XY ESCs (Wutz et al., 2002 PMID: 11780141)
- Organism: Mouse
- Growth properties: No disruption of Polycomb recruitment to the inactive X-chromosome.
- Model: Stem Cells
- Conditional: No
- Conditional description: Inducible through addition of doxycycline (1.5 ÄÂÄšĹg/ml) to the medium
- Description: Deletion of mouse Xist lncRNA to dissect specific RNA modules in the process of X-chromosome inactivation.
- Research area: Epigenetics & Nuclear Signalling; Stem Cell Biology
- Production details: CRISPR/Cas9 genome editing of the Xist-TetOP XY ESC line
- Additional notes: Cancer Research Technology Limited (trading research tools as Ximbio) has been granted a non-exclusive license to the CRISPR-Cas9 technology by ERS Genomics Ltd under the patent rights listed here. This license from ERS Genomics Ltd allows Ximbio to develop and commercialise CRISPR-Cas9 modified cell lines for research use only. Ximbio can provide these modified CRISPR-Cas9 cell lines to companies under a label-use only license.
- For Research Use Only
Target Details
- Target: Mouse Xist lncRNA
Application Details
Handling
- Format: Frozen
- Growth medium: ESCs grow in feeder-free classic ESC medium DMEM media containing 15% fetal bovine serum (FBS), 103 U/ml leukemia inhibitor factor (LIF), 10-4 mM 2-mercaptoethanol, 50 U/ml penicillin, and 50 ?g/ml of streptomycin (Gibco) at 37Ă°C in 5-8% CO2. Medium is changed daily Inducible expression of Xist driven by a TetO promoter is achieved by adding DOX (1.5 ?g/ml).
- Shipping conditions: Dry ice
Documentation
- Available on request
Related Tools
References
- • Bousard et al. 2019. EMBO Rep. 20(10):e48019. PMID: 31456285.
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