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Contributor Information

  • Name Jolanta Szenajch ; Luiza Handschuh ; Aleksandra Swiercz ; Michal GĂłralski ; Alicja Szabelska-Beresewicz ; Joanna Zyprych-Walczak ; Idzi Siatkowski
  • Institute Military Institute of Aviation Medicine

Tool Details

  • Tool name: A2780 PTX(64) resistant cell line
  • Tool type: Cell Lines
  • Tool sub-type: Continuous
  • Parental cell line: A2780
  • Organism: Human
  • Tissue: Ovary
  • Cancer type: Ovarian Cancer
  • Disease: Cancer
  • Description: The paclitaxel (PTX) resistant A2780 PTX (64) cell line with collateral sensitivity to cisplatin (CDDP) (inverse resistance) has been developed by continuous growing of A2780 PTX (32) cell line (cat. no 160732) in 64 nM PTX concentration (for details see Fig. 1). The half maximal inhibitory concentration (IC50) for PTX is about thirty seven times as high the IC50 for A2780, whereas for CDDP it is about eleven times lower (for details see Tab. 1). PTX and CDDP are standard chemotherapeutic drugs used in the treatment of ovarian cancer and acquired resistance to them remains a major obstacle in therapy. The trend of developing the inverse resistance is dominant in clinical practice, therefore A2780 PTX (64) cell line with other sublines of the series (see Notes for details) can be used as a primary tool for deciphering the molecular and cellular mechanisms of drug resistance in ovarian cancer.
  • Research area: Cancer ; Drug Discovery & Development

  • For Research Use Only

Target Details

Application Details

Handling

  • Format: Frozen
  • Growth medium: RPMI 1640 medium + 2mM L-alanine-L-glutamine (GlutaMAXTM) + 5% heat-inactivated Foetal Bovine Serum (FBS) , at 37°C and 5% CO2. To keep the stability of resistance profile not using antibiotics and antimycotics is recommended. Cells should be cultured without PTX (up to 10 days) to avoid the resistance escalation. After this time the maintenance treatment with 64 nM PTX for one passage is recommended.
  • Shipping conditions: Dry ice

Documentation

  • Available on request

References

  •   Szenajch et al. 2020. Int. J. Mol. Sci. 21(23): 9218. PMID: 33287223