CELL LINES
Contributor Information
- Name Maria Carmo-Fonseca ; SimĂÂo Teixeira da Rocha
- Institute Instituto de Medicina Molecular JoĂÂo Lobo Antunes
Tool Details
- Tool name: iPSC control for BRCA1-associated cancer model cell line (2040)
- Alternate names: iPSCs control 24 ÄËĂÂĂÂ clones 6 and 8 (IBBISTi2-A and IBBISTi2-B)
- Tool type: Cell Lines
- Parental cell line: Human fibroblasts
- Organism: Human
- Tissue: Fibroblast
- Disease: Hereditary breast cancer; Hereditary ovarian cancer; Control
- Model: Cancer Model
- Conditional: No
- Description: Control for BRCA1-associated cancer model. Investigation of tissue-specific stem/progenitor cells that represent the cells of origin of BRCA1-associated tumours, modelling early phases of malignant transformation and development of drug screening strategies aiming at preventing BRCA1 mutant hereditary cancers.
- Research area: Cancer; Gene Expression; Genetic Studies Tools; Stem Cell Biology
- Production details: Reprogramming of human induced pluripotent stem cells from skin biopsy of a familiar female healthy donor.
- For Research Use Only
Target Details
- Target: Healthy control
Application Details
Handling
- Format: Frozen
- Growth medium: Cells are cultured on MatrigelTM (Corning)-coated plates with mTeSRTMPlus Medium (StemCell Technologies). Medium is changed daily. Cells are passaged every 3ÄËĂÂĂÂ4 days (when the colonies covered approximately 85% of the surface area of the culture dish) using 0.5 mM EDTA dissociation buffer (Life Technologies). Cells are incubated at 37 ÄËĂÂÄšÂC in a humidified 5% CO2 incubator
- Shipping conditions: Dry ice
Documentation
- Available on request
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