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Contributor Information

  • Name Maria Carmo-Fonseca ; Simăo Teixeira da Rocha
  • Institute Instituto de Medicina Molecular Joăo Lobo Antunes

Tool Details

  • Tool name: iPSC control for BRCA1-associated cancer model cell line (2040)
  • Alternate names: iPSCs control 24 – clones 6 and 8 (IBBISTi2-A and IBBISTi2-B)
  • Tool type: Cell Lines
  • Parental cell line: Human fibroblasts
  • Organism: Human
  • Tissue: Fibroblast
  • Disease: Hereditary breast cancer; hereditary ovarian cancer; control
  • Model: Cancer Model
  • Conditional: No
  • Description: Control for BRCA1-associated cancer model. Investigation of tissue-specific stem/progenitor cells that represent the cells of origin of BRCA1-associated tumours, modelling early phases of malignant transformation and development of drug screening strategies aiming at preventing BRCA1 mutant hereditary cancers.
  • Research area: Cancer ; Gene Expression ; Genetic Studies Tools ; Stem Cell Biology
  • Production details: Reprogramming of human induced pluripotent stem cells from skin biopsy of a familiar female healthy donor.

  • For Research Use Only

Target Details

  • Target: Healthy control

Application Details

Handling

  • Format: Frozen
  • Growth medium: Cells are cultured on MatrigelTM (Corning)-coated plates with mTeSRTMPlus Medium (StemCell Technologies). Medium is changed daily. Cells are passaged every 3–4 days (when the colonies covered approximately 85% of the surface area of the culture dish) using 0.5 mM EDTA dissociation buffer (Life Technologies). Cells are incubated at 37 ◦C in a humidified 5% CO2 incubator
  • Shipping conditions: Dry ice

Documentation

  • Available on request