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Contributor Information

  • Institute Boyce Thompson Institute

Tool Details

  • Tool name: Tnao38 subclone cell line
  • Alternate names: BTI-Tnao38
  • Tool type: Cell Lines
  • Parental cell line: High Five (T. ni)
  • Growth properties: Flat fibroblast-like appearance at lower densities and more spherical appearance at higher cell densities and in suspension.
  • Conditional: Yes
  • Conditional description: Tnao38 is a HighFive (Trichoplusia ni) sub-clone accidentally isolated from a primary culture of Ascalapha odarata cells
  • Description: The insect cell based baculovirus expression system is a well established and widely used system for the production of recombinant proteins A Trichoplusia ni derived insect cell line shown to be highly productive, in both quality and quantity, in the expression of recombinant proteins via the baculovirus expression system. Tnao38 cells have been shown to be exceed protein expression of both Sf9 and High Five cell lines (Hashimoto et al.,2010). Absence of alphanodavirus based on RT-PCR (Hashimoto et al., 2010) Link to original HighFive clone confirmed by SNP
  • Research area: Gene Expression ; Other
  • Production details: Thawing Procedure: Remove cells from liquid nitrogen and thaw quickly in a 37°C water bath. Centrifuge gently (10Âş C, 800 rpm, 5 min) to pellet cells. Resuspend cell pellet in pre-warmed (27°C) medium and transfer to T25 flask. Grow at 27°C.
  • Cellosaurus ID: CVCL_Z252

  • For Research Use Only

Target Details

Application Details

Handling

  • Format: Frozen
  • Growth medium: TNMFH+10%FBS [1] in T25, T75, or T150 flasks at 27°C
  • Shipping conditions: Dry ice
  • Initial handling information: Thawing Procedure: Remove cells from liquid nitrogen and thaw quickly in a 37°C water bath. Centrifuge gently (10ş C, 800 rpm, 5 min) to pellet cells. Resuspend cell pellet in pre-warmed (27°C) medium and transfer to T25 flask. Grow at 27°C. Doubling time: 20.2 hrs in adherent cell culture ( see Hashimoto et al. 2010 ). Passage when ~90% confluent (Dislodge)

Documentation

  • Available on request