CELL LINES
Contributor Information
- Name Medical-Industrial Translational Research Center
- Institute Fukushima Medical University
Tool Details
- Tool name: RKID001 Kidney cancer organoid
- Alternate names: RKID001, RKID1
- Tool type: Cell Lines
- Tool sub-type: Organoids
- Organism: Human
- Tissue: Kidney
- Gender: Female
- Cancer type: Kidney
- Growth properties: Suspension
- Application: 3D cell culture
- Description: A series of novel patient-derived organoids (PDOs) have been constructed from different tumor tissue types under the Fukushima Translational Research Project, designated as F-PDO. F-PDOs form large cell clusters with a morphology similar to the original tumor and can be cultured for more than six months. Our comparative histological and comprehensive gene expression analyses have shown that the characteristics of F-PDOs were similar to their source tumors, even after long-term growth in culture conditions
- Research area: Cancer
- For Research Use Only
Target Details
Application Details
- Application: 3D cell culture
Handling
- Format: Frozen
- Passage number: 5
- Growth medium: Cancer Cell Expansion Media plus (Fujifilm Wako Pure Chemical, Ltd.) with 10 ng/ml bFGF, 10 ľM Y27632, 30 ng/ml EGF, and 1 ľM A8301.
- Temperature: 37C
- Atmosphere: 5% CO2
- Volume: 1 ml
- Storage medium: CELLBANKER 2
- Storage conditions: Liquid Nitrogen
- Shipping conditions: Dry ice
- Characterisation tests: Pathogen and stelility test; Comprehensive gene expression analysis; Cancer panel sequencing; Whole exome sequencing
- Mycoplasma free: Yes
- Biosafety level: 1
Documentation
- Available on request
User manual
Brochure
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