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Contributor Information

  • Name Motoki Takagi
  • Institute Fukushima Medical University
  • Primary citation Higa et al. 2021. Journal of visualized experiments. PMID: 34180908

Tool Details

  • Tool name: RLUN007_3 Lung cancer organoid
  • Alternate names: RLUN007-3, RLUN7-3, RLUN7, RLUN007
  • Tool type: Cell Lines
  • Tool sub-type: Organoids
  • Organism: Human
  • Tissue: Lung
  • Cancer type: Lung cancer, Adenocarcinoma in situ
  • Growth properties: Suspension
  • Application: 3D cell culture, High-throughput screening
  • Description: A series of novel patient-derived organoids (PDOs) have been constructed from different tumor tissue types under the Fukushima Translational Research Project, designated as F-PDO. F-PDOs form large cell clusters with a morphology similar to the original tumor and can be cultured for more than six months. Our comparative histological and comprehensive gene expression analyses have shown that the characteristics of F-PDOs were similar to their source tumors, even after long-term growth in culture conditions
  • Research area: Cancer

  • For Research Use Only

Target Details

Application Details

  • Application: 3D cell culture, High-throughput screening

Handling

  • Format: Frozen
  • Growth medium: Cancer Cell Expansion Media plus (Fujifilm Wako Pure Chemical, Ltd.) with 1% B-27 Supplement and 30 ng/ml EGF.
  • Temperature: 37C
  • Atmosphere: 5% CO2
  • Storage medium: CELLBANKER 2
  • Storage conditions: Liquid Nitrogen
  • Shipping conditions: Dry ice
  • Quality control: Pathogen and stelility test, Comprehensive gene expression analysis, Cancer panel sequencing, Whole exome sequencing, Anticancer drug susceptibility testing
  • Mycoplasma free: Yes
  • Biosafety level: 1

Documentation

    Download Icon User manual
    Download Icon Brochure
  • Available on request

References

  •   Higa et al. 2021. Journal of visualized experiments. PMID: 34180908