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Contributor Information

  • Name Medical-Industrial Translational Research Center
  • Institute Fukushima Medical University

Tool Details

  • Tool name: RLUN008-2 Lung cancer organoid
  • Alternate names: RLUN008-2, RLUN8-2, RLUN8, RLUN008
  • Tool type: Cell Lines
  • Tool sub-type: Organoids
  • Organism: Human
  • Donor: 63 years old female patient
  • Tissue: Lung
  • Gender: Female
  • Cancer type: Lung cancer
  • Growth properties: Suspension
  • Application: 3D cell culture, High-throughput screening
  • Description: A series of novel patient-derived organoids (PDOs) have been constructed from different tumor tissue types under the Fukushima Translational Research Project, designated as F-PDO. F-PDOs form large cell clusters with a morphology similar to the original tumor and can be cultured for more than six months. Our comparative histological and comprehensive gene expression analyses have shown that the characteristics of F-PDOs were similar to their source tumors, even after long-term growth in culture conditions
  • Research area: Cancer

  • For Research Use Only

Target Details

Application Details

  • Application: 3D cell culture, High-throughput screening


  • Format: Frozen
  • Passage number: 5
  • Growth medium: Cancer Cell Expansion Media plus (Fujifilm Wako Pure Chemical, Ltd.).
  • Temperature: 37C
  • Atmosphere: 5% CO2
  • Volume: 1 ml
  • Storage medium: CELLBANKER 2
  • Storage conditions: Liquid Nitrogen
  • Shipping conditions: Dry ice
  • Characterisation tests: Pathogen and stelility test; Comprehensive gene expression analysis; Cancer panel sequencing; Whole exome sequencing; Anticancer drug susceptibility testing
  • Mycoplasma free: Yes
  • Biosafety level: 1


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