CELL LINES
Contributor Information
- Name Amy Chadwick
- Institute University of Liverpool, Molecular and Clinical Pharmacology
Tool Details
- Tool name: UOL-Hep-CYB7 cell line
- Tool type: Cell Lines
- Parental cell line: HepG2
- Organism: Human
- Tissue: Liver
- Disease: Mitochondrial Subhaplogroup : J1c2h
- Growth properties: Adherent
- Model description: Cybrid Cell Line
- Description: This cell line consists of HepG2 cybrid cell lines negative for cellular mitochondrial DNA, fused with different platelet partners (platelets contain only mitochondrial DNA and no nuclear DNA). Each cybrid cell line has a combination of SNPs in mitochondrial DNA, relevant to a variety of diseases. Cybrid cell line panel has potential use for drug testing
- Research area: Drug Discovery & Development; Drug Safety Testing
- Additional notes: Parental cell line was purchased from ECACC
- For Research Use Only
Target Details
Application Details
- Application notes: SNPs characteristic of assigned haplogroup : 185A 188G 222T 228A 263G 295T 462T 489C 750G 1438G 3010A 4216C 4769G 7028T 8860G 10398G 11251G 11719A 12612G 13708A 14766T 14798C 15326G 15452A 16069T 16126C Additional SNPs: 16189C 16519C 16527T
Handling
- Growth medium: DMEM high-glucose medium (glucose; 25?mM) supplemented with FBS (10 % v/v), L-glutamine (4?mM), sodium pyruvate (1?mM), and HEPES (1?mM)
- Temperature: 37C
- Atmosphere: 5% CO2
- Subculture routine: Cells are subcultured weekly (1:12) via trypsinisation.
Documentation
- Available on request
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