CELL LINES
Contributor Information
- Name Amy Chadwick
- Institute University of Liverpool, Molecular and Clinical Pharmacology
Tool Details
- Tool name: UOL-HepCYB10 cell line
- Tool type: Cell Lines
- Parental cell line: HepG2
- Organism: Human
- Tissue: Liver
- Disease: Mitochondrial Subhaplogroup : H2a1e1a1
- Growth properties: Adherent
- Model description: Cybrid Cell Line
- Description: This cell line consists of HepG2 cybrid cell lines negative for cellular mitochondrial DNA, fused with different platelet partners (platelets contain only mitochondrial DNA and no nuclear DNA). Each cybrid cell line has a combination of SNPs in mitochondrial DNA, relevant to a variety of diseases. Cybrid cell line panel has potential use for drug testing
- Research area: Drug development
- Additional notes: Parental cell line was purchased from ECACC
- For Research Use Only
Target Details
Application Details
- Application notes: SNPs characteristic of assigned haplogroup : 263G 575T 750G 751G 951A 8860G 15326G 16124C 16148T 16166G 16354T
Handling
- Growth medium: DMEM high-glucose medium (glucose; 25?mM) supplemented with FBS (10 % v/v), L-glutamine (4?mM), sodium pyruvate (1?mM), and HEPES (1?mM)
- Temperature: 37C
- Atmosphere: 5% CO2
- Subculture routine: Cells are subcultured weekly (1:12) via trypsinisation.
Documentation
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