CELL LINES
Contributor Information
- Name Amy Chadwick
- Institute University of Liverpool, Molecular and Clinical Pharmacology
Tool Details
- Tool name: UOL-Hep-Rho0 cell line
- Tool type: Cell Lines
- Parental cell line: HepG2
- Organism: Human
- Tissue: Liver
- Growth properties: Adherent
- Model description: HepG2 Rho0 cell line (devoid of mitochondrial DNA)
- Description: This cell line consists of HepG2 cybrid cell lines negative for cellular mitochondrial DNA, fused with different platelet partners (platelets contain only mitochondrial DNA and no nuclear DNA). Each cybrid cell line has a combination of SNPs in mitochondrial DNA, relevant to a variety of diseases. Cybrid cell line panel has potential use for drug testing
- Research area: Drug Discovery & Development; Drug Safety Testing
- Additional notes: Parental cell line was purchased from ECACC
- For Research Use Only
Target Details
Application Details
- Application notes: No measurable mitochondrial DNA
Handling
- Growth medium: DMEM/F-12 + GlutaMAXTM supplemented with FBS (10 % v/v), L-glutamine (4?mM), sodium pyruvate (1?mM), HEPES (2?mM) and uridine (500 ?M)
- Subculture routine: Cells are subcultured as required (1:6), via trypsinisation, but media is changed twice weekly
Documentation
- Available on request
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