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Contributor Information

  • Name T.R. Santhosh Kumar
  • Institute Rajiv Gandhi Centre for Biotechnology
  • Primary citation Srinivas KP, et.al. PMID:26992219

Tool Details

  • Tool name: MDA-MB-231/EGFP_Cytochrome c
  • Tool type: Cell Lines
  • Parental cell line: MDA-MB-231
  • Organism: Human
  • Tissue: Breast
  • Gender: Female
  • Cancer type: Breast cancer
  • Disease: Cancer
  • Morphology: Epithelial
  • Growth properties: Adherent
  • Model: Transgenic
  • CRISPR: No
  • Conditional: No
  • Description: The product is a human cervical carcinoma cell line MDA-MB-231, transfected to stably express the protein cytochrome c (cyt-c) tagged with EGFP. The EGFP-tag enables easy identification of the cyt-c release in live cells upon apoptotic induction. The cell line can be utilized for studying the apoptosis process and to screen of potential candidate drugs that induce apoptosis.
  • Research area: Cancer, Neurodegenerative diseases, Metabolic disorders

  • For Research Use Only

Target Details

Application Details

Handling

  • Growth medium: DMEM with 2mM L-Glutamine and 10% FBS (Heat inactivated), or Leibovitz's L15 with 2mM L-Glutamine and 10% Fetal Bovine serum.
  • Temperature: 37C
  • Atmosphere: 5% CO2
  • Storage medium: Growth medium + 10% DMSO
  • Storage conditions: Liquid Nitrogen
  • Shipping conditions: Dry Ice
  • Initial handling information: Resuscitation: Rapidly thaw the frozen ampoule in a water bath at 37°C for 1-2 minutes. Transfer the contents to a centrifuge tube and slowly add 5-10ml of pre-warmed growth media. Remove a sample for counting. Centrifuge at 100-150g for 2-3 minutes to pellet cells (a centrifugation step to remove the cryoprotectant is essential. Seed at the recommend density, e.g. greater than 10,000 cells per cm2.
  • Subculture routine: Subculture non-confluent cultures using trypsin/EDTA and 1:3 to 1:4, i.e. seeding at 1-4 x10,000 cells/cm˛ .
  • Cultured in antibiotics?: No

Documentation

  • Available on request

References

  •   Srinivas KP, et.al. PMID:26992219