CELL LINES
Contributor Information
- Name T.R. Santhosh Kumar
- Institute Rajiv Gandhi Centre for Biotechnology
Tool Details
- Tool name: HEK 293/EGFP_ ACE2
- Tool type: Cell Lines
- Parental cell line: HEK 293
- Organism: Human
- Tissue: Other
- Gender: Female
- Cancer type: Other
- Disease: Cancer
- Morphology: Epithelial
- Growth properties: Adherent
- Model: Transgenic
- CRISPR: No
- Conditional: No
- Description: SARS CoV2 permissive cell line HEK 293 validated for Spike protein binding and SARS CoV 2 pseudovirion assay
- Research area: Cancer; Neurodegenerative diseases; Metabolic disorders
- For Research Use Only
Target Details
Application Details
Handling
- Growth medium: DMEM with 2mM L-Glutamine and 10% FBS (Heat inactivated).
- Temperature: 37C
- Atmosphere: 5% CO2
- Storage medium: Growth medium + 10% DMSO
- Storage conditions: Liquid Nitrogen
- Shipping conditions: Dry Ice
- Initial handling information: Resuscitation: Rapidly thaw the frozen ampoule in a water bath at 37°C for 1-2 minutes. Transfer the contents to a centrifuge tube and slowly add 5-10ml of pre-warmed growth media. Remove a sample for counting. Centrifuge at 100-150g for 2-3 minutes to pellet cells (a centrifugation step to remove the cryoprotectant is essential. Seed at the recommend density, e.g. greater than 10,000 cells per cm2.
- Subculture routine: Subculture non-confluent cultures using trypsin/EDTA and 1:3 to 1:4, i.e. seeding at 1-4 x10,000 cells/cmË .
- Cultured in antibiotics?: No
Documentation
- Available on request
Related Tools
References
- • Unpublished
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