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Contributor Information

  • Name Medical-Industrial Translational Research Center
  • Institute Fukushima Medical University
  • Primary citation Hoshi et al. Oncol Rep. 2017 Jan, 37(1):66-76. PMID: 27840973

Tool Details

  • Tool name: MCF10A-EGFR1m-Control cell line
  • Alternate names: 06M01001
  • Clone: EGFR-1_Control
  • Tool type: Cell Lines
  • Tool sub-type: Continuous
  • Parental cell line: MCF10A, a non-tumorigenic cell line from human mammarygland epithelium
  • Organism: Human
  • Tissue: Mammarygland epithelium
  • Cancer type: Non-tumorigenic
  • Morphology: Epithelial
  • Growth properties: Adherent
  • Model description: Control
  • Application: Functional analysis of mutated genes, Drug screening
  • Description: Non-tumorigenic immortalized breast epithelial cell stably overexpressing mutant cancer-related genes. EGFR (epidermal growth factor receptor). EGFR is a trans-membrane type receptor tyrosine kinase and signaling caused by EGFR is thought to play a crucial role in maintaining homeostasis of normal tissues by being involved in the regulation of cells such as proliferation and differentiation. Mutation of EGFR gene is found in lung cancer and it is known that its constant activation causes cell proliferation and tumorigenesis. Deletion of the Exon19 region, mutation of L858R and T790M are known as somatic cell mutations often found in cancer.
  • Research area: Cancer; Cell biology

  • For Research Use Only

Target Details

  • Target: Epidermal growth factor receptor [EGFR]
  • Target background: Gene ID: 1956; References: DNA (mRNA): NM_005228.3; Protein: NP_005219.2

Application Details

  • Application: Functional analysis of mutated genes, Drug screening

Handling

  • Format: Frozen
  • Growth medium: DMEM/Ham's F-12 supplemented with 5% heat-inactivated horse serum, 10 Â?g/ml insulin (human, recombinant), 5 Â?M forskolin, 0.5 Â?g/ml hydrocortisone, 20 ng/ml EGF (human, recombinant), 100 U/ml penicillin, and 100 Â?g/ml streptomycin
  • Temperature: 37?C
  • Atmosphere: Humidified incubator with 5% CO2
  • Storage medium: CELLBANKER 2 (Zenogen pharma)
  • Storage conditions: Liquid Nitrogen
  • Shipping conditions: Dry ice
  • Characterisation tests: Comprehensive gene expression analysis; Identification of cell proliferation and cellular form; Drug sensitivity tests
  • Initial handling information: Thaw the vial at 37 °C in a water bath?then seed cells into a T75 flask. Culture in a humidified Incubator at 37 ? with 5% CO2 in air. Culture medium should be changed every 2 to 3 days.
  • Cultured in antibiotics?: 100 U/ml Penicillin, and 100 Â?g/ml Streptomycin
  • Mycoplasma free: Yes
  • Biosafety level: 1

Documentation

References

  •   Hoshi et al. Oncol Rep. 2017 Jan, 37(1):66-76. PMID: 27840973