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MCF10A-EGFR1m-3 cell line

Cat. #161170

Tool name: MCF10A-EGFR1m-3 cell line
Alternate names: 06M01012
Clone: EGFR-1_type-07
Tool type: Cell Lines
Tool sub-type: Continuous
Parental cell line: MCF10A, a non-tumorigenic cell line from human mammarygland epithelium
Organism: Human
Tissue: Mammarygland epithelium
Cancer type: Non-tumorigenic
Morphology: Epithelial
Growth properties: Adherent
Model: Mutant
Model description: Type-07; Type mutation: L861Q; CDS mutation: c.2582T>A; AA mutation: p.L861Q; COSMIC Mutation ID: COSM6213
Application: Functional analysis of mutated genes, Drug screening
Description: Non-tumorigenic immortalized breast epithelial cell stably overexpressing mutant cancer-related genes. EGFR (epidermal growth factor receptor).Â EGFR is a trans-membrane type receptor tyrosine kinase and signaling caused by EGFR is thought to play a crucial role in maintaining homeostasis of normal tissues by being involved in the regulation of cells such as proliferation and differentiation.Â Mutation of EGFR gene is found in lung cancer and it is known that its constant activation causes cell proliferation and tumorigenesis. Deletion of the Exon19 region, mutation of L858R and T790M are known as somatic cell mutations often found in cancer.
Research area: Cancer; Cell biology

Target: Epidermal growth factor receptor [EGFR]
Target background: Gene ID: 1956; References: DNA (mRNA): NM_005228.3; Protein: NP_005219.2

Format: Frozen
Growth medium: DMEM/Ham's F-12 supplemented with 5% heat-inactivated horse serum, 10 Âľg/ml insulin (human, recombinant), 5 ÂľM forskolin, 0.5 Âľg/ml hydrocortisone, 20 ng/ml EGF (human, recombinant), 100 U/ml penicillin, and 100 Âľg/ml streptomycin
Temperature: 37?C
Atmosphere: Humidified incubator with 5%Â CO2
Storage medium: CELLBANKER 2 (Zenogen pharma)
Storage conditions: Liquid Nitrogen
Shipping conditions: Dry ice
Characterisation tests: Comprehensive gene expression analysis; Identification of cell proliferation and cellular form; Drug sensitivity tests
Initial handling information: Thaw the vial at 37 Â°C in a water bath?then seed cells into a T75 flask. Culture in a humidified Incubator at 37 ? with 5% CO2 in air. Culture medium should be changed every 2 to 3 days.
Cultured in antibiotics?: 100 U/ml Penicillin, and 100 Âľg/ml Streptomycin
Mycoplasma free: Yes
Biosafety level: 1

Quantity:

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