MCF10A-ERRB2m-9 cell line

Tool Details

*FOR RESEARCH USE ONLY

• Name: MCF10A-ERRB2m-9 cell line
• Alternate name: 06M15004
• Tool sub type: Continuous
• Parental cell: MCF10A, a non-tumorigenic cell line from human mammarygland epithelium
• Clone: ERBB2_type-10
• Organism: Human
• Tissue: Mammarygland epithelium
• Morphology: Epithelial
• Growth properties: Adherent
• Model: Mutant
• Model description: Type-10; Type mutation: V777L; CDS mutation: c.2329G>T; AA mutation: p.V777L; COSMIC Mutation ID: COSM14062
• Description: Non-tumorigenic immortalized breast epithelial cell stably overexpressing mutant cancer-related gene, ERBB2 (erb-B2 Receptor Tyrosine Kinase 2). ERBB2 is a member of the epidermal growth factor (EGF) receptor family of receptor tyrosine kinases. This protein bind tightly to other ligand-bound EGF receptor family members to form a heterodimer, stabilizing ligand binding and enhancing kinase-mediated activation of downstream signalling pathways. Allelic variations at amino acid positions 654 and 655 of isoform a (positions 624 and 625 of isoform b) have been reported, with the most common allele, Ile654/Ile655. Amplification and/or overexpression of this gene has been reported in numerous cancers, including breast and ovarian tumors.
• Application: Functional analysis of mutated genes, Drug screening
• Biosafety level: 1

Target Details

• Target: Erb-b2 receptor tyrosine kinase 2 [ERBB2]
• Target background: Gene ID: 2064; References: DNA (mRNA): NM_004448.3; Protein: NP_004439.2

Applications

• Application: Functional analysis of mutated genes, Drug screening

Handling

• Format: Frozen
• Growth medium: DMEM/Ham's F-12 supplemented with 5% heat-inactivated horse serum, 10 Â?g/ml insulin (human, recombinant), 5 Â?M forskolin, 0.5 Â?g/ml hydrocortisone, 20 ng/ml EGF (human, recombinant), 100 U/ml penicillin, and 100 Â?g/ml streptomycin
• Temperature: 37° C
• Atmosphere: Humidified incubator with 5% CO2
• Shipping conditions: Dry ice
• Storage medium: CELLBANKER 2 (Zenogen pharma)
• Storage conditions: Liquid Nitrogen
• Initial handling information: Thaw the vial at 37 °C in a water bath?then seed cells into a T75 flask. Culture in a humidified Incubator at 37 ? with 5% CO2 in air. Culture medium should be changed every 2 to 3 days.
• Cultured in antibiotics: 100 U/ml Penicillin, and 100 Â?g/ml Streptomycin
• Mycoplasma free: Yes
• Characterisation tests: Comprehensive gene expression analysis; Identification of cell proliferation and cellular form; Drug sensitivity tests

References

• Irie et al. Mol Cancer Ther. 2019 Apr, 18(4):733-742. PMID: 30787176