CELL LINES
Contributor Information
- Name Medical-Industrial Translational Research Center
- Institute Fukushima Medical University
Tool Details
- Tool name: MCF10A-PTENm-5 cell line
- Alternate names: 06M17013
- Clone: PTEN_type-12
- Tool type: Cell Lines
- Tool sub-type: Continuous
- Parental cell line: MCF10A, a non-tumorigenic cell line from human mammarygland epithelium
- Organism: Human
- Tissue: Mammarygland epithelium
- Cancer type: Non-tumorigenic
- Morphology: Epithelial
- Growth properties: Adherent
- Model: Mutant
- Model description: Type-12; Type mutation: R335*; CDS mutation: c.1003C>T; AA mutation: p.R335*; COSMIC Mutation ID: -
- Application: Functional analysis of mutated genes, Drug screening
- Description: Non-tumorigenic immortalized breast epithelial cell stably overexpressing mutant cancer-related gene, PTEN (phosphatase and tensin homolog). PTEN is a multi-functional tumor suppressor that is mutated and lost in a large number of cancers at high frequency. Observed in prostate cancer, glioblastoma, endometrial, lung and breast cancer to varying degrees. Up to 70% of prostate cancer patients have been observed to have loss of expression of the gene. It is a part of the PI3K/AKT/mTOR pathway and mTOR inhibitors have been relatively ineffective in treating patients with PTEN loss.
- Research area: Cancer; Cell biology
- For Research Use Only
Target Details
- Target: Phosphatase and tensin homolog [PTEN]
- Target background: Gene ID: 5728; References: DNA (mRNA): NM_000314.6; Protein: NP_000305.3
Application Details
- Application: Functional analysis of mutated genes, Drug screening
Handling
- Format: Frozen
- Growth medium: DMEM/Ham's F-12 supplemented with 5% heat-inactivated horse serum, 10 µg/ml insulin (human, recombinant), 5 µM forskolin, 0.5 µg/ml hydrocortisone, 20 ng/ml EGF (human, recombinant), 100 U/ml penicillin, and 100 µg/ml streptomycin
- Temperature: 37?C
- Atmosphere: Humidified incubator with 5% CO2
- Storage medium: CELLBANKER 2 (Zenogen pharma)
- Storage conditions: Liquid Nitrogen
- Shipping conditions: Dry ice
- Characterisation tests: Comprehensive gene expression analysis; Identification of cell proliferation and cellular form; Drug sensitivity tests
- Initial handling information: Thaw the vial at 37 °C in a water bath?then seed cells into a T75 flask. Culture in a humidified Incubator at 37 ? with 5% CO2 in air. Culture medium should be changed every 2 to 3 days.
- Cultured in antibiotics?: 100 U/ml Penicillin, and 100 µg/ml Streptomycin
- Mycoplasma free: Yes
- Biosafety level: 1
Documentation
- Available on request
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