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Contributor Information

  • Name William G Watson
  • Institute University College Dublin
  • Primary citation O'Neill et al. Mol Cancer. 2011 Oct 7;10:126. PMID: 21982118

Tool Details

  • Tool name: PC-3 D12 cell line
  • Alternate names: PC-3 Docetaxel resistant cell line
  • Tool type: Cell Line
  • Organism: Human
  • Tissue: Pancreas
  • Cancer type: Pancreatic Cancer
  • Disease: Cancer
  • Growth properties: Suspension
  • Model: Mutant
  • CRISPR: No
  • Products or characteristics of interest: Docetaxel resistant cell line
  • Application: Mechanisms of resistance to Docetaxe in pancreatic cancer cells
  • Description: Docetaxel resistant sub-lines were generated in the androgen-independent (PC-3, DU-145) and sensitive (22RV1) cell lines. One of the main mechanisms of resistance to Docetaxel is the over expression of P-gp which would reduce intracellular concentrations of the drug through increased efflux. Chemoresistant PC-3 cells do not over express P-gp. We demonstrated that the resistance in the PC-3 sublines was independent of P-gp over expression as there were no detectable levels and no effects of the P-gp inhibitor Elacridar on their resistance
  • Research area: Cancer
  • Production details: PC-3 resistant sub-lines were generated by initially treating with Docetaxel (Sigma) at 4 nM and 8 nM (suspended in dimethyl sulfoxide (DMSO) (Fluke)) in 75 cm2 flasks for 48 hours. After treatment, the surviving cells were re-seeded into new flasks and allowed to recover for 2-3 weeks. After 5 (at 8 nM) and 7 (at 4 nM) treatments, the dose of docetaxel was increased from 4 nM and 8 nM to 8 nM and 12 nM respectively. The cells underwent a total of 18 treatment cycles at 8 nM and 12 treatment cycles at 12 nM, (cell passage numbers 21-55). Following each treatment they were allowed to fully recover before assessing their resistance to docetaxel and any experimental work.

  • For Research Use Only

Target Details

Application Details

  • Application: Mechanisms of resistance to Docetaxe in pancreatic cancer cells

Handling

  • Format: Frozen
  • Growth medium: RPMI-1640 medium supplemented with 10% Fetal Bovine Serum (FBS), 50 U/ml penicillin/50 ?g/ml streptomycin and 2 mM L-glutamine
  • Storage conditions: Liquid Nitrogen
  • Shipping conditions: Dry Ice

Documentation

  • Available on request

References

  •   O'Neill et al. Mol Cancer. 2011 Oct 7
  •   10:126. PMID: 21982118