1.2B4 cell line

Tool Details

*FOR RESEARCH USE ONLY (for other uses, please contact the licensing team)

• Name: 1.2B4 cell line
• Research fields: Disease (not cancer)
• Tool sub type: Hybrid cell line
• Parental cell: HuP-T3 cell line
• Organism: Human
• Tissue: Pancreas
• Disease: Diabetes
• Morphology: Epitheloid
• Growth properties: Adherent
• Model: Transgenic
• Model description: Hybrid cell line formed by the electrofusion of a primary culture of human pancreatic islets with HuP-T3, a human pancreatic carcinoma cell line
• Crispr: No
• Description: A hybrid cell line formed by the electrofusion of a primary culture of human pancreatic islets with HuP-T3, a human pancreatic carcinoma cell line. 1.2B4 has been shown to be tumourigenic when transplanted into a SCID mouse host.
• Application: producing pure insulin secreting cells when stimulated
• Additional notes: Patent: Flatt P & McClenaghan N (2004) Insulin producing cell-line US patent number 20040005702 (A1). Also WO0210346 (A2).

Applications

• Application: producing pure insulin secreting cells when stimulated

Handling

• Format: Frozen
• Growth medium: RPMI-1640 + 2mM Glutamine + 10% FCS
• Temperature: 37° C
• Atmosphere: 5% CO2
• Shipping conditions: Dry Ice
• Storage conditions: Liquid Nitrogen
• Initial handling information: Split sub-confluent cultures (70-80%) 1:2 to 1:6 i.e. seeding at 2-4 x 104 cells/cmÂ? using 0.05% trypsin or trypsin/EDTA; 5% CO2; 37°C. Population doubling approx 20hrs. At confluence 105 cells/cmÂ? can be expected.
• Mycoplasma free: Yes
• Str profiling: STR-PCR Data:Amelogenin: X,YCSF1PO: 10D13S317: 9,11D16S539: 10,13D5S818: 10,14D7S820: 12THO1: 9TPOX: 8,12vWA: 18

References

• Guo-Parke H et al. 2012. J Endocrinol. 214(3):257-65. PMID: 22685334