CELL LINES
Contributor Information
- Name Peter Flatt and Neville McClenaghan
- Institute Ulster University
- Primary citation Nishimura N et. al. 1993. Int J Pancreatol 13: 31-41 PMID: 8454916.
Tool Details
- Tool name: 1.2B4 cell line
- Tool type: Cell Line
- Tool sub-type: Hybrid cell line
- Parental cell line: HuP-T3 cell line
- Organism: Human
- Tissue: Pancreas
- Disease: Diabetes
- Morphology: Epitheloid
- Growth properties: Adherent
- Model: Transgenic
- Model description: Hybrid cell line formed by the electrofusion of a primary culture of human pancreatic islets with HuP-T3, a human pancreatic carcinoma cell line
- CRISPR: No
- Application: producing pure insulin secreting cells when stimulated
- Description: A hybrid cell line formed by the electrofusion of a primary culture of human pancreatic islets with HuP-T3, a human pancreatic carcinoma cell line. 1.2B4 has been shown to be tumourigenic when transplanted into a SCID mouse host.
- Research area: Disease (not cancer)
- Additional notes: Patent: Flatt P & McClenaghan N (2004) Insulin producing cell-line US patent number 20040005702 (A1). Also WO0210346 (A2).
- For Research Use Only
Target Details
Application Details
- Application: producing pure insulin secreting cells when stimulated
Handling
- Format: Frozen
- Growth medium: RPMI-1640 + 2mM Glutamine + 10% FCS
- Temperature: 37°C
- Atmosphere: 5% CO2
- Storage conditions: Liquid Nitrogen
- Shipping conditions: Dry Ice
- Initial handling information: Split sub-confluent cultures (70-80%) 1:2 to 1:6 i.e. seeding at 2-4 x 104 cells/cm� using 0.05% trypsin or trypsin/EDTA; 5% CO2; 37°C. Population doubling approx 20hrs. At confluence 105 cells/cm� can be expected.
- STR profile: STR-PCR Data: Amelogenin: X,Y CSF1PO: 10 D13S317: 9,11 D16S539: 10,13 D5S818: 10,14 D7S820: 12 THO1: 9 TPOX: 8,12 vWA: 18
- Mycoplasma free: Yes
Documentation
Related Tools
References
- • Guo-Parke H et al. 2012. J Endocrinol. 214(3):257-65. PMID: 22685334
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