CELL LINES
Contributor Information
- Name Michelle Lockley
- Institute Queen Mary University of London
- Primary citation J I Hoare et al. 2022. Gynecol Oncol. 167(1):96-106. PMID: 35918200
Tool Details
- Tool name: CovCis cell line
- Tool type: Cell Lines
- Parental cell line: Cov318 cell line
- Organism: Mouse
- Tissue: Ovary
- Gender: Female
- Cancer type: Gynaecologic cancer
- Growth properties: Adherent
- Model: Mutant
- Model description: Platinum-resistant HGSC in vivo cell line
- CRISPR: No
- Description: Cells show increased expression of the ABCB1 drug efflux pump as well as reversion mutations in BRCA genes and loss of BRCA promoter-methylation. Platinum-resistant HGSC cancer cells with a growth advantage in the presence of therapy that expand to create drug-resistant tumours
- Research area: Cancer
- Production details: Cells were cultured in vitro in increasing concentrations of either cisplatin or carboplatin until an increase in IC50 between two and ten-fold was achieved to reflect the resistance observed in human patients
- For Research Use Only
Target Details
Application Details
Handling
- Growth medium: DMEM containing 10% FBS and 1% penicillin/streptomycin
- Temperature: 37C
- Cultured in antibiotics?: Streptomycin and Penicillin
- STR profile: 16 locus STR verification (DNA Diagnostics Centre, London, UK: June 2015?February 2016 and European Collection of Authenticated Cell Lines August 2019)
- Mycoplasma free: Yes
Documentation
Related Tools
References
- • J I Hoare et al. 2022. Gynecol Oncol. 167(1):96-106. PMID: 35918200
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