Product Image

Contributor Information

  • Name Ralf H. Adams
  • Institute Cancer Research UK, London Research Institute: Lincoln's Inn Fields

Tool Details

  • Tool name: Bmx Cre ERT2 Mouse
  • Tool type: Experimental models
  • Tool sub-type: Mouse
  • Disease: Cancer; Angiogenesis
  • Model: Conditional KO
  • Conditional: Yes
  • Conditional description: Conditional Cre-ERT2 expression under Bmx promoter enabling tissue-specific recombinase in arterial endothelial cells; inducible Cre activity by treatment with hormone (tamoxifen) enabling inducible translocation of Cre-ERT2 to nucleus.
  • Genetic background and cross history: A Bmx-Cre-ERT2 transgene vector, containing a genomic VECad promoter fragment fused to a Cre-ERT2 cDNA, was injected into fertilised embryos (C57BL/6 or FVB/N). Founder lines were back-crossed to establish mice heterozygous for the Bmx-Cre-ERT2 transgene.
  • Phenotype:
  • Zygosity: Heterozygous
  • Description: The estragen receptor (ERT2) under the Bone marrow x (Bmx) promoter (Bmx-Cre-ERT2) mouse exhibits tissue-specific expression of an inducible Cre-ERT2 fusion protein, enabling tamoxifen-induced Cre recombinase activity in arterial endothelial cells. The Bmx-Cre-ERT2 mouse is an ideal tool in the study of gene function in angiogenesis, atherosclerosis and neovascularisation. Administration of tamoxifen induces nuclear translocation of the Cre-ERT2 fusion protein, and subsequent Cre recombinase activity, allowing knockout/knockin/transgene studies of loxP flanked genes in endothelial cells.Non-induced Bmx-Cre-ERT2 mice demonstrate no Cre recombinase activity, while tamoxifen-induced Bmx-Cre-ERT2 mice demonstrate high penetrance in endothelial cells (95%+), significantly higher than existing endothelial Cre models currently available.
  • Research area: Developmental Biology; Genetic studies; Immunology; Neurobiology
  • Production details: A Bmx-Cre-ERT2 transgene vector, containing a genomic VECad promoter fragment fused to a Cre-ERT2 cDNA, was injected into fertilised embryos (C57BL/6 or FVB/N). Founder lines were back-crossed to establish mice heterozygous for the Bmx-Cre-ERT2 transgene.
  • Additional notes:

  • For Research Use Only

Target Details

  • Target: BMX, CreERT2

Application Details

Handling

  • Shipping conditions: Embryo/Spermatoza- Dry Ice

Documentation

    Download Icon
    Download Icon
    Download Icon
    Download Icon
  • Available on request

Related Tools

Product Image

p110beta PI3K-D931A (129)

#158388
Product Image

SAR-22-097F mouse line

#161681
Product Image

FH1 KO Mouse

#151657

References

  • •  Murphy et al. 2014. Proc Natl Acad Sci U S A. 111(50):18007-12. PMID: 25468970.
  • •  Constitutively active Notch4 receptor elicits brain arteriovenous malformations through enlargement of capillary-like vessels.
  • •  Noels et al. 2014. Arterioscler Thromb Vasc Biol. 34(6):1209-20. PMID: 24723559.
  • •  Schober et al. 2014. Nat Med. 20(4):368-76. PMID: 24584117.
  • •  Deficiency of endothelial CXCR4 reduces reendothelialization and enhances neointimal hyperplasia after vascular injury in atherosclerosis-prone mice.
  • •  MicroRNA-126-5p promotes endothelial proliferation and limits atherosclerosis by suppressing Dlk1.
  • •  Ehling et al. 2013. Development. 140(14):3051-61. PMID: 23785053.
  • •  Notch controls retinal blood vessel maturation and quiescence.