EXPERIMENTAL MODELS
Contributor Information
- Name Fiona Watt
- Institute Cancer Research UK, London Research Institute: Lincoln's Inn Fields
Tool Details
- Tool name: Integrin a6b4 Mouse
- Tool type: Experimental models
- Tool sub-type: Mouse
- Cancer type: Squamous cell carcinoma
- Disease: Cancer
- Conditional: Yes
- Conditional description: Conditional expression of integrins under involucrin promoter giving tissue-specific suprabasal epidermal expression.
- Cell signalling pathway: Integrin Mediated Signalling
- Genetic background and cross history: Involucrin - integrin transgene expression constructs were injected into fertilized oocytes from F1 hybrid CBAxC57Bl/6 mice. Transgene-positive mice were back-crossed to generate individual founder lines. alpha6 founder line was crossed with beta4 founder line to generate alpha6beta4 line.
- Phenotype: Mice present phenotype very similar to human psoriasis disease.
- Zygosity: Heterozygous
- Strain:
- Description: Chemically-induced skin tumourigenesis model; in vivo study of alpha6 and beta4 integrin transgene expression in skin. Ninety six percent of alpha6beta4 transgenic mice develop squamous cell carcinoma, compared to 44% wildtype. Of those mice developing tumours, 68% alpha6beta4 develop metastasis, compared to 8% wildtype. TGF-beta signalling is disrupted in alpha6beta4 transgenic mice.
- Research area: Cancer ; Cell Type or Organelle Marker ; Cell Signaling & Signal Transduction ; Genetic studies ; Stem Cell Biology
- Production details: Involucrin - integrin transgene expression constructs were injected into fertilized oocytes from F1 hybrid CBAxC57Bl/6 mice. Transgene-positive mice were back-crossed to generate individual founder lines. alpha6 founder line was crossed with beta4 founder line to generate alpha6beta4 line.
- Additional notes:
- For Research Use Only
Target Details
- Target: Alpha6Beta4 Integrin
Application Details
Handling
- Shipping conditions: Embryo/Spermatoza- Dry Ice
Documentation
- Available on request
References
- • Owens DM et al, Journal of Cell Science, 2003, v116 pp3783-3791PMID: 12902406
.svg)
Availability: 
.svg)
.svg)
