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Contributor Information

  • Name Fiona Watt
  • Institute Cancer Research UK, London Research Institute: Lincoln's Inn Fields

Tool Details

  • Tool name: Integrin a6b4 Mouse
  • Tool type: Experimental models
  • Tool sub-type: Mouse
  • Cancer type: Squamous cell carcinoma
  • Disease: Cancer
  • Conditional: Yes
  • Conditional description: Conditional expression of integrins under involucrin promoter giving tissue-specific suprabasal epidermal expression.
  • Cell signalling pathway: Integrin Mediated Signalling
  • Genetic background and cross history: Involucrin - integrin transgene expression constructs were injected into fertilized oocytes from F1 hybrid CBAxC57Bl/6 mice. Transgene-positive mice were back-crossed to generate individual founder lines. alpha6 founder line was crossed with beta4 founder line to generate alpha6beta4 line.
  • Phenotype: Mice present phenotype very similar to human psoriasis disease.
  • Zygosity: Heterozygous
  • Strain:
  • Description: Chemically-induced skin tumourigenesis model; in vivo study of alpha6 and beta4 integrin transgene expression in skin. Ninety six percent of alpha6beta4 transgenic mice develop squamous cell carcinoma, compared to 44% wildtype. Of those mice developing tumours, 68% alpha6beta4 develop metastasis, compared to 8% wildtype. TGF-beta signalling is disrupted in alpha6beta4 transgenic mice.
  • Research area: Cancer ; Cell Type or Organelle Marker ; Cell Signaling & Signal Transduction ; Genetic studies ; Stem Cell Biology
  • Production details: Involucrin - integrin transgene expression constructs were injected into fertilized oocytes from F1 hybrid CBAxC57Bl/6 mice. Transgene-positive mice were back-crossed to generate individual founder lines. alpha6 founder line was crossed with beta4 founder line to generate alpha6beta4 line.
  • Additional notes:

  • For Research Use Only

Target Details

  • Target: Alpha6Beta4 Integrin

Application Details

Handling

  • Shipping conditions: Embryo/Spermatoza- Dry Ice

Documentation

  • Available on request

References

  •   Owens DM et al, Journal of Cell Science, 2003, v116 pp3783-3791PMID: 12902406