EXPERIMENTAL MODELS
Contributor Information
- Name Tomas Lindahl
- Institute Cancer Research UK, London Research Institute: Clare Hall Laboratories
Tool Details
- Tool name: UNG Mouse
- Tool type: Experimental models
- Tool sub-type: Mouse
- Cancer type: B cell lymphoma
- Disease: Cancer
- Conditional: No
- Conditional description:
- Genetic background and cross history: A ung targeting vector, replacing exon 4 with a resistance cassette, was transfected into 129 ES cells. Properly targeted ES cells containing a homologous recombination event were selected, cloned, and injected into C57BL6 blastocysts. Chimeric mice were mated with C57BL6 mice to generate heterozygotes. Heterozygous mice were crossed to generate homozygous Ung-/- mice.
- Phenotype:
- Zygosity: Homozygous
- Description: In vivo study of UNG knockout and DNA mismatch mutation during DNA synthesis. Mice develop B cell lymphoma and are abnormally defective in their immune response. There are also mouse embryonic fibroblasts from this line available.
- Research area: Cancer; DNA Damage and Repair; Genetic studies; Immunology
- Production details: A ung targeting vector, replacing exon 4 with a resistance cassette, was transfected into 129 ES cells. Properly targeted ES cells containing a homologous recombination event were selected, cloned, and injected into C57BL6 blastocysts. Chimeric mice were mated with C57BL6 mice to generate heterozygotes. Heterozygous mice were crossed to generate homozygous Ung-/- mice.
- Additional notes:
- For Research Use Only
Target Details
- Target: Uracil-DNA Glycosylase (UNG)
Application Details
Handling
- Shipping conditions: Embryo/Spermatoza- Dry Ice
Documentation
- Available on request
References
- • Nilsen et al. 2003. Oncogene. 22(35):5381-6. PMID: 12934097.
- • Gene-targeted mice lacking the Ung uracil-DNA glycosylase develop B-cell lymphomas.
- • Nilsen et al. 2000. Mol Cell. 5(6):1059-65. PMID: 10912000.
- • Uracil-DNA glycosylase (UNG)-deficient mice reveal a primary role of the enzyme during DNA replication.
.svg)
Availability: 
.svg)
.svg)
