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Contributor Information

  • Name Ralf H. Adams
  • Institute Cancer Research UK, London Research Institute: Lincoln's Inn Fields

Tool Details

  • Tool name: JamC -/- Mouse
  • Tool type: Experimental models
  • Tool sub-type: Mouse
  • Disease: Neuropathy; inflammatory disease;
  • Model: Knock-Out
  • Conditional: No
  • Conditional description:
  • Genetic background and cross history: A JamC targeting vector, containing loxP flanked exons 4, 5, and a cDNA encoding exons 6 to 9, an exon 4 - reporter fusion, and a frt flanked resistance cassette, was injected into E14 ES cells. Properly targeted ES cells containing a homologous recombination event were selected, cloned, and injected into C57BL6 blastocysts. Chimeric offspring were mated to C57BL6 mice, and floxed JamC lines maintained on a mixed 129/C57BL6 background. Floxed JamC mice were crossed with Cre expressing mice to generate heterozygous JamC+/- mice. Heterozygous JamC+/- mice were interbred to generate JamC-/- mice.
  • Phenotype: Mice are maintained as heterozygotes (JamC+/-) to permit breeding (due to male fertility defects in JamC-/- mice).
  • Zygosity: Heterozygous
  • Strain:
  • Description: In vivo study of JAM-C knockout; in vivo study of spermiogenesis; in vivo study of neuronal networks and integrity
  • Research area: Cancer ; Cell Signaling & Signal Transduction ; Genetic studies ; Neurobiology
  • Production details: A JamC targeting vector, containing loxP flanked exons 4, 5, and a cDNA encoding exons 6 to 9, an exon 4 - reporter fusion, and a frt flanked resistance cassette, was injected into E14 ES cells. Properly targeted ES cells containing a homologous recombination event were selected, cloned, and injected into C57BL6 blastocysts. Chimeric offspring were mated to C57BL6 mice, and floxed JamC lines maintained on a mixed 129/C57BL6 background. Floxed JamC mice were crossed with Cre expressing mice to generate heterozygous JamC+/- mice. Heterozygous JamC+/- mice were interbred to generate JamC-/- mice.
  • Additional notes: Mice are maintained as heterozygotes (JamC+/-) to permit breeding (due to male fertility defects in JamC-/- mice).

  • For Research Use Only

Target Details

  • Target: Junctional adhesion molecule-C (JAM-C)

Application Details

Handling

  • Shipping conditions: Embryo/Spermatoza- Dry Ice

Documentation

  • Available on request

References

  •   Gliki et al. 2004. Nature. 431(7006):320-4. PMID: 15372036.
  •   Spermatid differentiation requires the assembly of a cell polarity complex downstream of junctional adhesion molecule-C.