EXPERIMENTAL MODELS
Contributor Information
- Name Ralf H. Adams
- Institute Cancer Research UK, London Research Institute: Lincoln's Inn Fields
Tool Details
- Tool name: JamC -/- Mouse
- Tool type: Experimental models
- Tool sub-type: Mouse
- Disease: Neuropathy; inflammatory disease;
- Model: Knock-Out
- Conditional: No
- Conditional description:
- Genetic background and cross history: A JamC targeting vector, containing loxP flanked exons 4, 5, and a cDNA encoding exons 6 to 9, an exon 4 - reporter fusion, and a frt flanked resistance cassette, was injected into E14 ES cells. Properly targeted ES cells containing a homologous recombination event were selected, cloned, and injected into C57BL6 blastocysts. Chimeric offspring were mated to C57BL6 mice, and floxed JamC lines maintained on a mixed 129/C57BL6 background. Floxed JamC mice were crossed with Cre expressing mice to generate heterozygous JamC+/- mice. Heterozygous JamC+/- mice were interbred to generate JamC-/- mice.
- Phenotype: Mice are maintained as heterozygotes (JamC+/-) to permit breeding (due to male fertility defects in JamC-/- mice).
- Zygosity: Heterozygous
- Strain:
- Description: In vivo study of JAM-C knockout; in vivo study of spermiogenesis; in vivo study of neuronal networks and integrity
- Research area: Cancer ; Cell Signaling & Signal Transduction ; Genetic studies ; Neurobiology
- Production details: A JamC targeting vector, containing loxP flanked exons 4, 5, and a cDNA encoding exons 6 to 9, an exon 4 - reporter fusion, and a frt flanked resistance cassette, was injected into E14 ES cells. Properly targeted ES cells containing a homologous recombination event were selected, cloned, and injected into C57BL6 blastocysts. Chimeric offspring were mated to C57BL6 mice, and floxed JamC lines maintained on a mixed 129/C57BL6 background. Floxed JamC mice were crossed with Cre expressing mice to generate heterozygous JamC+/- mice. Heterozygous JamC+/- mice were interbred to generate JamC-/- mice.
- Additional notes: Mice are maintained as heterozygotes (JamC+/-) to permit breeding (due to male fertility defects in JamC-/- mice).
- For Research Use Only
Target Details
- Target: Junctional adhesion molecule-C (JAM-C)
Application Details
Handling
- Shipping conditions: Embryo/Spermatoza- Dry Ice
Documentation
- Available on request
References
- • Gliki et al. 2004. Nature. 431(7006):320-4. PMID: 15372036.
- • Spermatid differentiation requires the assembly of a cell polarity complex downstream of junctional adhesion molecule-C.
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