EXPERIMENTAL MODELS
Contributor Information
- Name Fiona Watt
- Institute Cancer Research UK, London Research Institute: Lincoln's Inn Fields
Tool Details
- Tool name: K14dNLef1 Mouse
- Tool type: Experimental models
- Tool sub-type: Mouse
- Cancer type: Sebaceous adenoma; Sebeoma; Squamous papilloma
- Disease: Cancer; Sebaceous adenoma; Sebeoma; Squamous papilloma; Telogen effluvium;
- Conditional: Yes
- Conditional description: Conditional expression of deltaN-Lef1 under Keratin 14 promoter giving tissue-specific epidermal expression.
- Genetic background and cross history: A deltaNLef1 transgene vector, containing an N-terminally deleted mouse Lef1 cDNA under the control of the keratin 14 promoter, was injected into fertilised CBA/C57BL6 ooctyes. Founders were backcrossed to establish transgenic lines.
- Phenotype: Tumourigenesis (skin); skin abnormalities (hair follicle, sebaceous gland)
- Zygosity:
- Description: In vivo study of Lef1 activity in skin; model for hair growth & follicle formation; disease model for skin tumour formation (sebaceous adenoma, sebeoma, squamous papilloma)
- Research area: Cancer; Cell Signaling & Signal Transduction; Developmental Biology; Epigenetics & Nuclear Signalling; Genetic studies; Stem Cell Biology
- Production details: A deltaNLef1 transgene vector, containing an N-terminally deleted mouse Lef1 cDNA under the control of the keratin 14 promoter, was injected into fertilised CBA/C57BL6 ooctyes. Founders were backcrossed to establish transgenic lines.
- Additional notes:
- For Research Use Only
Target Details
- Target: Lef1 N-terminal mutant (constituitive transcirptional activity)
Application Details
Handling
- Shipping conditions: Embryo/Spermatoza- Dry Ice
Documentation
- Available on request
References
- • Niemann et al. 2002. Development. 129(1):95-109. PMID: 11782404.
- • Expression of DeltaNLef1 in mouse epidermis results in differentiation of hair follicles into squamous epidermal cysts and formation of skin tumours.
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