Product Image

Contributor Information

  • Name Nic Jones
  • Institute Cancer Research UK, Manchester Institute

Tool Details

  • Tool name: ATF2 Mouse
  • Tool type: Experimental models
  • Tool sub-type: Mouse
  • Conditional: No
  • Genetic background and cross history: Atf2 genomic sequences were isolated by screening a 129/ola mouse cosmid library (RZPD), cloned into pPGKneobpAlox2PGKDTA, transfected into 129/ola embryonic stem cells. Transgenic animals were produced by blastocyst injection and offspring mice were backcrossed at least six times with C57 Bl/6 mice to produce isogenic lines.
  • Phenotype: Homozygous postnatal lethality, heterozygous normal phenotype
  • Zygosity: Heterozygous
  • Description: Alanine substitutions of phosphor acceptor sites T51, T53 (equivalent to human T69, T71). Transcription factor (BZIP-type, AP1-family)
  • Research area: Cell Cycle ; Cell Signaling & Signal Transduction ; Developmental Biology ; Epigenetics & Nuclear Signalling ; Genetic studies ; Immunology
  • Production details: Atf2 genomic sequences were isolated by screening a 129/ola mouse cosmid library (RZPD), cloned into pPGKneobpAlox2PGKDTA, transfected into 129/ola embryonic stem cells. Transgenic animals were produced by blastocyst injection and offspring mice were backcrossed at least six times with C57 Bl/6 mice to produce isogenic lines.

  • For Research Use Only

Target Details

  • Target: ATF2

Application Details

Handling

  • Shipping conditions: Embryo/Spermatoza- Dry Ice

Documentation

  • Available on request

References

  •   Breitwieser et al. 2007. Genes Dev. 21(16):2069-82. PMID: 17699753.
  •   Feedback regulation of p38 activity via ATF2 is essential for survival of embryonic liver cells.