EXPERIMENTAL MODELS
Contributor Information
- Name Martin Turner
- Institute Babraham Institute
Tool Details
- Tool name: p110Delta KO Mouse
- Alternate names: PI3Kdelta Phosphatidylinositol-4,5-Bisphosphate 3-Kinase 11 KDa Catalytic Subunit Delta phosphoinositide-3-kinase C
- Tool type: Experimental models
- Tool sub-type: Mouse
- Disease: Germinal Center Reaction
- Model description:
- Zygosity: Homozygous
- Description: p110δ is a member of the PI3K family that phosphorylates phosphoinositides on the 3-hydroxyl group of the inositol ring. The PI3K pathway has been implicated in lymphocyte development and activation. p110δ plays a role in oncogenic transformation, and is consistently expressed at a high level in blast cells from AML. Additionally, p110δ signaling pathway is involved in immune responses relevant to the pathogenesis of rheumatoid arthritis and other inflammatory diseases.
- Research area: Cell biology; Cell signaling and signal transduction; Genetics; Immunology
- Production details: LoxP flanked neomycin and hygromycin-resistance cassettes were cloned 7.5 kb apart into the EcoRV and XhoI sites respectively of a murine pik3cd genomic isolated from a 129/Sv genomic library. The targeting vector was transfected into PC3 mouse embryonic stem cells and analyzed for the targeting event using Southern blotting of KpnI-digested DNA yielding a 700-bp EcoRI fragment. Correctly targeted clones were injected into blastocysts to produce chimaeric mice. The resulting chimaeras, which express the Cre enzyme in the male germline, were bred to obtain lines of mice harboring a pik3cd gene which had undergone Cre-mediated recombination and thus deleted exons 1â9 encoding the first 490 amino acids of p110δ. These were detected by Southern blot analysis using KpnI-digested DNA and by PCR.
- Breeding information: p110δ-deficient mice are born in normal Mendelian ratios from heterozygous intercrosses and are fertile and healthy under SPF conditions.
- For Research Use Only
Target Details
- Target: p110δ
Application Details
Handling
Documentation
Related Tools
References
- • Clayton et al. 2002. J Exp Med. 196(6):753-63. PMID: 12235209
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