- Name Roland Wolf
- Institute University of Dundee
- Tool name: HRN Mouse
- Tool type: Experimental models
- Tool sub-type: Mouse
- Genetic background and cross history: The HRN model was created by targeting the Por gene to generate a floxed allele in GK129/1 embryonic stem cells derived from 129P2 mice and injecting the targeted cells into C57BL/6 blastocysts. Resultant chimeras were backcrossed to C57BL/6 for one generation. Mice heterozygous for the floxed Por allele were intercrossed to generate mice homozygous for the floxed Por allele on a mixed B6;129P2 background. The Alb-cre transgene was developed in the laboratory of Mark A. Magnuson at Vanderbilt University School of Medicine by microinjecting Cre recombinase gene under the control of the rat albumin enhancer/promoter into B6D2F2 zygotes. Mice homozygous for the floxed Por allele were bred to carriers for the Alb-cre transgene to generate HRN mice.
- Phenotype: Hepatic Cytochrome P450 Reductase Null
- Zygosity: Homozygous
- Strain: C57BL/6
- Description: The HRN mouse model lacks P450 activity in the liver and can be used to study the role of hepatic P450 in drug metabolism and to determine the efficacy or toxicity of pharmacological compounds. HRN mice are homozygous for the floxed Por allele and the Alb-Cre transgene. POR is the electron donating enzyme for all of cytochrome P450 enzymes.
- Research area: Cancer ; Drug Discovery & Development ; Genetic studies ; Metabolism
- For Research Use Only
- • Henderson et al. 2003. J Biol Chem. 278(15):13480-6. PMID: 12566435.
- • Inactivation of the hepatic cytochrome P450 system by conditional deletion of hepatic cytochrome P450 reductase.