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Contributor Information

  • Name Michael Olson
  • Institute Cancer Research UK, Glasgow: The Beatson Institute

Tool Details

  • Tool name: Hprt-CAG-LSL-ROCK2:ER Mouse
  • Alternate names: Rho-associated protein kinase 2, Rho-associated coiled-coil-containing protein kinase 2, ROCK-II, Hypoxanthine phosphoribosyltransferase, HPRT, CAG, chicken actin gene,
  • Tool type: Experimental models
  • Tool sub-type: Mouse
  • Model: Transgenic
  • Conditional: Yes
  • Conditional description: Injection of tamoixfen activates excision of the LSL (loxP-stop-loxP) by Cre recombinase and enables expression of GFP:ROCK2 fusion protein
  • Application: This strain can be crossed with mice expressing Cre recombinase under the transcriptional control of tissue specific promoters to restrict GFP:ROCK2:ER expression to tissues of choice.
  • Zygosity: Heterozygous
  • Strain: C57BL/6
  • Description: Rho associated coiled-coil containing protein kinases (ROCKs) exist in mammals, zebrafish, Xenopus, C. elegans and Drosophila. They are mainly involved in regulating the shape and movement of cells through acting on the cytoskeleton. Two mouse ROCK isoforms ROCK1 and ROCK2 have been identified. ROCK1 is mainly expressed in the lung, liver, spleen, kidney and testis and ROCK2 is distributed mostly in the brain and heart. ROCK2, an isoenzyme of ROCK1, is a serine/threonine kinase and regulates cytokinesis, smooth muscle contraction, the formation of actin stress fibers and activation of the c-fos serum response element.
  • Research area: Cancer; Cell biology; Genetics
  • Additional notes: This strain can be crossed with mice expressing Cre recombinase under the transcriptional control of tissue specific promoters to restrict GFP:ROCK2:ER expression to tissues of choice.

  • For Research Use Only

Target Details

  • Target: Hprt gene-targeted Cre-inducible, tissue-specific expression of GFP:ROCK2

Application Details

  • Application: This strain can be crossed with mice expressing Cre recombinase under the transcriptional control of tissue specific promoters to restrict GFP:ROCK2:ER expression to tissues of choice.

Handling

  • Shipping conditions: Embryo/Spermatoza- Dry Ice

Documentation

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References

  • •  Tissue-selective expression of a conditionally-active ROCK2-estrogen receptor fusion protein.
  • •  Samuel et al. 2016. Genesis. :. PMID: 27775859.