- Name Chris Marshall
- Institute The Institute of Cancer Research
- Tool name: ROCK2 Floxed Mouse
- Alternate names: ROCK2; Rho-associated protein kinase 2; Rho-associated, coiled-coil-containing protein kinase 2; ROCK-II
- Tool type: Experimental models
- Tool sub-type: Mouse
- Model: Transgenic
- Conditional: Yes
- Conditional description: Requires expression of Cre recombinase to mediate excision of the lox P sites generating a non-functional ROCK 2 protein
- Cell signalling pathway: RHOA-ROCK signalling; Integrin mediated signalling
- Genetic background and cross history: Lox P sequences were inserted to flank exons 5 and 6 of the ROCK 2 gene
- Zygosity: Homozygous
- Strain: C57BL/6
- Description: Rho associated coiled-coil containing protein kinases (ROCKs) exist in mammals, zebrafish, Xenopus, C. elegans and Drosophila. They are mainly involved in regulating the shape and movement of cells through acting on the cytoskeleton. Two mouse ROCK isoforms ROCK1 and ROCK2 have been identified. ROCK1 is mainly expressed in the lung, liver, spleen, kidney and testis and ROCK2 is distributed mostly in the brain and heart. ROCK1 and ROCK2 are downstream effectors of the Rho subfamily of small GTPases, activated by Rho GTPases. ROCK2, an isoenzyme of ROCK1, is a serine/threonine kinase and regulates cytokinesis, smooth muscle contraction, the formation of actin stress fibres and activation of the c-fos serum response element. ROCK1 is involved in cancer initiation and progression presumably through mechanisms related to metastasis, cell motility and angiogenesis.
- Research area: Cancer; Cell Cycle; Cell Structure and Motility; Cellular Transport; Developmental Biology
- Production details: Lox P sequences were inserted to flank exons 5 and 6 of the ROCK 2 gene
- Breeding information: Mice are viable and have a normal lifespan
- For Research Use Only
- • K
mper et al. 2016. Elife. 5:e12994. PMID: 26765561.
- • Rho-associated kinase (ROCK) function is essential for cell cycle progression, senescence and tumorigenesis.