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Contributor Information

  • Name Chris Marshall
  • Institute The Institute of Cancer Research

Tool Details

  • Tool name: RalA Floxed Mouse
  • Alternate names: RAS like proto-oncogene A, RALA, Ras-related protein Ral-A, RAL
  • Tool type: Experimental models
  • Tool sub-type: Mouse
  • Disease: Tumorigenesis
  • Model: Transgenic
  • Conditional description: Requires expression of Cre recombinase to mediate excision of the lox P sites generating a non-functional Ral A protein
  • Genetic background and cross history: Lox P sequences were inserted to flank exons 2 and 3 of the Ral A gene
  • Phenotype: Tumorigenesis
  • Zygosity: Heterozygous
  • Strain: C57BL/6
  • Description: Ras is a family of proteins which exist in all animal cell lineages and organs. The Ras protein family belongs to a group of proteins called small GTPases which are involved in cellular signal transduction. Ras activation, by incoming cellular signals, switches on other proteins which causes downstream activation of genes involved in cell growth, differentiation and survival. Mutations in Ras genes can lead to the production of permanently activated Ras proteins, overactive signalling inside the cell, even in the absence of incoming signals resulting in uncontrolled cell growth and division which can ultimately lead to cancer. RAL small GTPases, encoded by the Rala and Ralb genes, are members of the Ras superfamily and can act as downstream effectors of Ras. Although both proteins are highly similar, distinct functions have been identified. Ras-related protein Ral-A protein is encoded on chromosome 7, and has been implicated in insulin secretion, epithelial cell polarity neurite branching, neuronal polarity and GLUT4 translocation. Ras-related protein Ral-B protein RALB in migration/invasion, tumor cell survival, autophagy and TBK1 activation.
  • Research area: Cancer; Cell signaling and signal transduction; Developmental biology; Genetics; Neurobiology
  • Production details: Lox P sequences were inserted to flank exons 2 and 3 of the Ral A gene
  • Breeding information: Animals are healthy, fertile and have a normal life span

  • For Research Use Only

Target Details

  • Target: Ras-related protein Ral-A

Application Details

Handling

  • Shipping conditions: Embryo/Spermatoza- Dry Ice

Documentation

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References

  • •  Peschard et al. 2012. Curr Biol. 22(21):2063-8. PMID: 23063435.
  • •  Genetic deletion of RALA and RALB small GTPases reveals redundant functions in development and tumorigenesis.