EXPERIMENTAL MODELS
Contributor Information
- Name Gregory Riggins
- Institute Johns Hopkins University
Tool Details
- Tool name: IDH1 Mutant Glioma Xenograft (JHH-273) mouse
- Tool type: Experimental models
- Tool sub-type: Mouse
- Disease: Glial tumor formation
- Model: Mutant
- Genetic background and cross history: 4â6-week old female athymic nude mice
- Phenotype: 4â6-week old female athymic nude mice
- Description: An in vivo Isocitrate Dehydrogenase (IDH-1) glioma mouse xenograft model has been developed to further study effective therapies for IDH mutations characterized by increased DNA methylation and production of the common oncometabolite, 2-HG. Difficult grafting cell-culture techniques and current engineered cell line quality have limited additional laboratory studies; however, this model was developed directly from a WHO grade III- glial cell positive cancer patient tissue (JHH273) with a confirmed IDH mutation through DNA sequencing and maintains IDH-1 mutation features observed in human primary glial brain tumors. Additionally, when models were exposed to a demethlyating agent, 5-azacytidine, a reduction in methylation and tumor growth was observed opening the door for more clinical analysis. This model meets a critical need to test drug agents targeting mechanisms involved in glial tumor formation.
- Research area: Cancer ; Drug Discovery & Development
- For Research Use Only
Target Details
- Target: IDH1
Application Details
Handling
- Shipping conditions: Embryo/Spermatoza- Dry Ice
Documentation
- Available on request
Related Tools
References
- • Borodovsky et al. 2013. Oncotarget. 4(10):1737-47. PMID: 24077805.
.svg)
Availability: 
.svg)
.svg)
