EXPERIMENTAL MODELS
Contributor Information
- Name Bart Vanhaesebroeck
- Institute Ludwig Institute for Cancer Research
Tool Details
- Tool name: p110beta PI3K-D931A (129)
- Tool type: Experimental models
- Tool sub-type: Mouse
- Model: Knock-In
- Cell signalling pathway: PI3K/AKT signalling
- Genetic background and cross history: Knock-in mice in which the endogenous PIK3CB/p110beta PI3K gene is mutated so that it now encodes a p110beta protein with the D931A mutation in the ATP binding site, converting it to a kinase-dead p110beta protein which is expressed at the same level as wild-type p110beta. These mice have been backcrossed onto the sv129 background.
- Phenotype: Mice show prenatal partial lethality at at different embryonic stages (see PMID 26132308 for details). Homozygous male mice that are born are infertile with smaller than normal testicles. They cannot produce offspring.
- Research area: Cancer
- Production details: Knock-in mice in which the endogenous PIK3CB/p110beta PI3K gene is mutated so that it now encodes a p110beta protein with the D931A mutation in the ATP binding site, converting it to a kinase-dead p110beta protein which is expressed at the same level as wild-type p110beta. These mice have been backcrossed onto the sv129 background.
- Additional notes: Mice show prenatal partial lethality at at different embryonic stages (see PMID 26132308 for details). Homozygous male mice that are born are infertile with smaller than normal testicles. They cannot produce offspring.
- For Research Use Only
Target Details
- Target: PIK3CB
Application Details
Handling
- Shipping conditions: Embryo/Spermatoza- Dry Ice
Documentation
- Available on request
Related Tools
References
- • Guillermet-Guibert et al. 2015. PLoS Genet. 11(7):e1005304. PMID: 26132308.
- • Kulkarni et al. 2011. Sci Signal. 4(168):ra23. PMID: 21487106.
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