EXPERIMENTAL MODELS
Contributor Information
- Name Robert Zimmermann ; Franz Radner
- Institute University of Graz
Tool Details
- Tool name: MAGL-Flox mouse
- Alternate names: MGL, Mgll, Magl
- Tool type: Experimental models
- Tool sub-type: Mouse
- Model: Conditional KO
- Conditional: Yes
- Conditional description: Targeted, conditional ready
- Genetic background and cross history: These mice were generated using HM1-ES cells. ES cell clones were injected into C57BL/6J blastocysts to enable coat colour selection of the chimeras. Mice were subsequently backcrossed to C57BL/6J (âĽÂ 10 generations). When kept as a homozygous colony, mice are regularly backcrossed to C57BL/6J to avoid generation of subpopulations.
- Phenotype: These mice are conditional ready, and phenotypical identical to C57BL/6J mice.
- Zygosity: Homozygous
- Strain: C57BL/6J
- Description: Useful model for the generation of tissue/cell specific MAGL-deficient mice. Animals might be useful for studies, e.g. on energy metabolism or endocannabinoid signalling.
- Research area: Metabolism; Cell Signaling & Signal Transduction
- Production details: A floxed neomycin resistance cassette was inserted upstream of exon 3. An additional loxP site was inserted downstream of exon 4. Cre-mediated recombination removed the selection cassette leaving Mgll exon 3 and 4 floxed. Further details are available upon request.
- Breeding information: Good breeder. homozygous mice may be bred together to maintain a live colony.
- For Research Use Only
Target Details
- Target: Monoglyceride lipase; monoacyl glycerol lipase
Application Details
Handling
Documentation
- Available on request
Related Tools
References
- • 21454566
- • 26565024
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