PROTEINS
Contributor Information
- Name Piret Arukuusk ; Ly Porosk ; Ülo Langel
- Institute University of Tartu
Tool Details
- Tool name: NF704 peptide
- Alternate names: NickFect74
- Tool type: Proteins
- Tool sub-type: Synthetic Peptide
- Sequence: HHHHHHGO(ILLKALKALAKAIL)
- Cellular/ tissue localisation: Cell-penetrating peptide to assist delivery of siRNA into cells via endocytosis
- Source: Solid-phase synthesis
- Expression system: Synthetic
- Function: siRNA delivery
- Description: This peptide can be used to transport siRNA into cells. Use of cell-penetration peptides can increase the transport efficiency of extracellular synthetic nucleic acids, such as siRNAs, to help increase concentration of the siRNA at the desired cellular location. This peptide is based on NickFect55 (NF55) which is a peptide that can efficiently transport plasmid DNA into cells in vivo. To improve the efficiency of nucleic acid delivery, this peptide has been developed to improve the intracellular release mechanism resulting in higher local dosage of the cargo.
- Research area: Genetics
- Tag: stearoyl (18-carbon) fatty acid chain attached to the N-terminus of the peptide
- Purpose: siRNA delivery
- Additional notes: There is a stearoyl (18-carbon) fatty acid chain attached to the N-terminus of the peptide. The sequence in brackets is attached to the sidechain amino group rather than the a-amino group.
- For Research Use Only
Target Details
- Target: Other
Application Details
- Application notes: There is a stearoyl (18-carbon) fatty acid chain attached to the N-terminus of the peptide. The sequence in brackets is attached to the sidechain amino group rather than the Îą-amino group.
Handling
- Shipping conditions: Dry Ice
Documentation
Related Tools
References
- • Porosk et al. 2019. Biomater Sci. 7(10):4363-4374. PMID: 31411219.
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