Super pure Bovine Serum Albumin
- Name Andrzej Gamian ; Arkadiusz Bartys ; Magdalena Staniszewska ; Regina Danielewicz ; Katarzyna Dzierzba ; Agnieszka Szydelko ; Jadwiga Pietkiewicz
- Institute Polski O?rodek Rozwoju Technologii (PORT) Polish Center for Technology Development
Tool name: Super pure Bovine Serum Albumin
Alternate names: Bovine serum albumin, BSA
Tool type: Proteins
Tool sub-type: BSA
Expression system: Native
Description: Bovine serum albumin (BSA) is one of the least expensive, commercially available proteins. It is widely used as a model protein in pure research and a number of practical uses. BSA is also used in the construction of conjugated vaccines. After binding to polysaccharide antigens, it is possible to obtain effective and safe vaccines against pathogens. In a similar system, BSA is also used in neoplasm therapy. In such uses, BSA should have a high degree of purity. In particular, it should lack carbohydrate contamination. Earlier reports indicate the presence of high molecular weight derivatives in BSA preparations. Highly polymerized aggregates may form as a result of the reaction of the protein with reactive carbonyl compounds by way of glycation. The risk of the formation of such contaminating aggregates is possible due to the presence of reducing sugars in the protein environment.
Additionally, the presence of saccharide conjugates and high-molecular mass derivatives in available commercial BSA preparations excludes them from use in primary research in molecular biology. For this reason, it is necessary to obtain BSA in monomeric form and lacking saccharide inclusions.
Research area: Drug development
Animal free: No
- For Research Use Only
- • WO2013050830 - PURE ALBUMIN AND ITS METHOD OF PREPARATION AND DETECTION