Temperature sensitive Protease-V24
- Institute The University of Victoria
Tool name: Temperature sensitive Protease-V24
Alternate names: V24
Tool type: Proteins
Description: This novel temperature sensitive protease, a variant of subtilisin Carlsberg (SubC) protease, is expressed in Bacillus subtilis. Variant 24 (V24) have been shown to be capable of degrading a broad range of proteins in their native or denatured state.
V24 has an inactivation profile that allows protease digestion at 40Â°C and inactivation at 50Â°C. All activity is destroyed after 10 minutes of incubation at 50Â°C (See Figure above).
This variant has proven valuable in working with fragile specimen in which minimal heat treatment is desired. Due to the unique properties of V24 it has also been shown to improve molecular biology research, automated processes like Next-Gen Sequencing and disease diagnostics workflows.
No column purification or treatment at extreme temperatures are required to completely deactivate this variant.
This protease remains active in the presence of detergent such as SDS and Triton X-100.
Research area: Genetic Studies Tools
Additional notes: Canadian Patent Application No. 3028612
US Patent No. 10894954
- For Research Use Only
- • Thompson et al. 2020. FEMS Microbiol Lett. 367(19):. PMID: 33016320.