Temperature sensitive Protease-V25
- Institute The University of Victoria
Tool name: Temperature sensitive Protease-V25
Alternate names: V25
Tool type: Proteins
Description: This novel temperature sensitive protease, a variant of subtilisin Carlsberg (SubC) protease, is expressed in Bacillus subtilis . Variant 25 (V25) has been shown to be capable of degrading a broad range of proteins in their native or denatured state.
V25 has properties that allow it to digest a substrate at elevated temperatures in the 55Â°C range and still be deactivated at a moderate temperature in the range of 60-65Â°C. All activity is destroyed after a 10 minute incubation at 60Â°C (See Figure above.).
Due to the unique properties of V25 this variant has been shown to be useful in denaturing proteins in a complex mixture while retaining the ability to inactivate the enzyme below 65Â°C. It has also been shown to improve molecular biology research, automated processes like Next-Gen Sequencing and disease diagnostics workflows.
No column purification or treatment at extreme temperatures are required to completely deactivate this variant.
This protease remains active in the presence of detergent such as SDS and Triton X-100.
Research area: Genetics
Additional notes: Canadian Patent Application No. 3028612
US Patent No. 10894954
- For Research Use Only
- • Thompson et al. 2020. FEMS Microbiol Lett. 367(19):. PMID: 33016320.