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GST-tagged human cannabinoid CB1 receptor (ammino acids 414 to 472)

Cat. #161588

Name Gontzal García del Caño
Institute University of the Basque Country
Primary citation Saumell-Esnaola M et al. 2022. Microb Cell Fact. 21(1):192. PMID: 36109736.

Tool name: GST-tagged human cannabinoid CB1 receptor (ammino acids 414 to 472)
Tool type: Protein
Tool sub-type: GST fusion protein
Sequence: Saumell-Esnaola M et al. 2022. Microb Cell Fact. 21(1):192. PMID: 36109736.
Molecular weight of the target: 32828.56 Da
Expression system: Escherichia coli
Application: Protein standard for quantitative Western blot to determine CB1 receptor density in biological samples using antibodies raised against peptides that within the sequence spanning amino acids 414-472 at the C-terminus of the CB1 receptor. Protein-protein interaction assays (e.g., GST pull-down surface plasmon resonance) involving proteins interacting with the CB1 receptor at the sequence spanning amino acids 414-472 at the C-terminus of the CB1 receptor.
Description: Polypeptide spanning amino acids 414 to 472 (CB1414–472) of the cytosolic tail of the human cannaboinoid CB1 receptor fused to the C-terminus of the glutathione S-transferase (GST) tag. The linker between the GST tag and the CB1414–472 polyopeptide posses the consensus cleavage site LeuPheGlnGlyPro for the PreScission protease for removing the GST tag after purification.
Research area: Neurobiology; Cell biology; Molecular Biology; Biochemistry
Tag: Glutathione S-transferase (GST)
Additional notes: Contact the inventor for further information.

Application: Protein standard for quantitative Western blot to determine CB1 receptor density in biological samples using antibodies raised against peptides that within the sequence spanning amino acids 414-472 at the C-terminus of the CB1 receptor. Protein-protein interaction assays (e.g., GST pull-down surface plasmon resonance) involving proteins interacting with the CB1 receptor at the sequence spanning amino acids 414-472 at the C-terminus of the CB1 receptor.

Format: Solution
Purity: Affinity purified
Storage buffer: 125 mM Tris-HCl, 150 mM NaCl, 1 mM DTT, 1 mM EDTA, pH 7.4 Contains 50 mM reduced glutathione which may need to be removed (e.g. by dialysis) for certain assays.
Storage conditions: -80 oC
Shipping conditions: Frozen in dry ice

Application: Protein standard for quantitative Western blot to determine CB1 receptor density in biological samples using antibodies raised against peptides that within the sequence spanning amino acids 414-472 at the C-terminus of the CB1 receptor. Protein-protein interaction assays (e.g., GST pull-down surface plasmon resonance) involving proteins interacting with the CB1 receptor at the sequence spanning amino acids 414-472 at the C-terminus of the CB1 receptor.

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