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GST-tagged human cannabinoid CB1 receptor (amino acids 414 to 442)

Cat. #161589

Name Gontzal García del Caño
Institute University of the Basque Country
Primary citation Saumell-Esnaola M et al. 2022. Microb Cell Fact. 21(1):192. PMID: 36109736.

Tool name: GST-tagged human cannabinoid CB1 receptor (amino acids 414 to 442)
Tool type: Protein
Tool sub-type: GST fusion protein
Sequence: Saumell-Esnaola M et al. 2022. Microb Cell Fact. 21(1):192. PMID: 36109736.
Molecular weight of the target: 29710.86 Da
Expression system: Escherichia coli
Application: Negative control for quantitative Western blot to determine CB1 receptor density in biological samples using antibodies raised against peptides within the sequence spanning amino acids 443-472 at the C-terminus of the CB1 receptor. Negative control in protein-protein interaction assays (e.g., GST pull-down surface plasmon resonance) involving proteins interacting with the CB1 receptor at the sequence spanning amino acids 414-472 of the C-terminal end of the CB1 receptor.
Description: Polypeptide spanning amino acids 414 to 442 (CB1414–442) of the cytosolic tail of the human cannaboinoid CB1 receptor fused to the C-terminus of the glutathione S-transferase (GST) tag. The linker between the GST tag and the CB1414–442 polyopeptide posses the consensus cleavage site LeuPheGlnGlyPro for the PreScission protease for removing the GST tag after purification.
Research area: Neurobiology; Cell biology; Molecular Biology; Biochemistry
Tag: Glutathione S-transferase (GST)
Additional notes: Contact the inventor for further information.

Application: Negative control for quantitative Western blot to determine CB1 receptor density in biological samples using antibodies raised against peptides within the sequence spanning amino acids 443-472 at the C-terminus of the CB1 receptor. Negative control in protein-protein interaction assays (e.g., GST pull-down surface plasmon resonance) involving proteins interacting with the CB1 receptor at the sequence spanning amino acids 414-472 of the C-terminal end of the CB1 receptor.

Format: Solution
Purity: Affinity purified
Storage buffer: 126 mM Tris-HCl, 150 mM NaCl, 1 mM DTT, 1 mM EDTA, pH 7.4 Contains 50 mM reduced glutathione which may need to be removed (e.g. by dialysis) for certain assays.
Storage conditions: -80 oC
Shipping conditions: Frozen in dry ice

Application: Negative control for quantitative Western blot to determine CB1 receptor density in biological samples using antibodies raised against peptides within the sequence spanning amino acids 443-472 at the C-terminus of the CB1 receptor. Negative control in protein-protein interaction assays (e.g., GST pull-down surface plasmon resonance) involving proteins interacting with the CB1 receptor at the sequence spanning amino acids 414-472 of the C-terminal end of the CB1 receptor.

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