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Contributor Information

  • Name Gontzal García del Caño
  • Institute University of the Basque Country
  • Primary citation Contact the inventor for further information.

Tool Details

  • Tool name: Top10 E. coli for ampicillin-resistant vector propogation of GST and human CB1 receptor (AA 414-472 ) fusion protein
  • Tool type: Bacteria
  • Tool sub-type: Transformed One Shot™ TOP10 E. coli
  • Sequence: Saumell-Esnaola M et al. 2022. Microb Cell Fact. 21(1):192. PMID: 36109736.
  • Application: Production of the IPTG-inducible plasmid pGEX with the sequence encoding the GST-CB1414–472 fusion protein.
  • Description: E. coli strain for the production of plasmid DNA carrying ampicillin resistance and containing the IPTGinducible coding sequence for the polypeptide spanning amino acids 414 to 472 of the cytosolic tail of the human CB1 receptor (CB1414–472) fused to the C-terminus of the glutathione S-transferase (GST) tag.
  • Research area: Neurobiology; Cell biology; Molecular Biology; Biochemistry

  • For Research Use Only

Target Details

Application Details

  • Application: Production of the IPTG-inducible plasmid pGEX with the sequence encoding the GST-CB1414–472 fusion protein.

Handling

  • Format: Stock Glycerol
  • Storage conditions: -80 oC
  • Shipping conditions: Frozen in dry ice

Documentation

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References

  • •  Gómez-Caballero et al. 2021. Mikrochim Acta. 188(11):368. PMID: 34618242.