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Contributor Information

  • Name Gontzal García del Caño
  • Institute University of the Basque Country
  • Primary citation Contact the inventor for further information.

Tool Details

  • Tool name: Top10 E. coli for kanamycin-resistent vector propagation of GST and human CB1 receptor (AA 414 to 442)
  • Tool type: Bacteria
  • Tool sub-type: Transformed One Shot™ TOP10 E. coli
  • Sequence: Saumell-Esnaola M et al. 2022. Microb Cell Fact. 21(1):192. PMID: 36109736.
  • Application: Production of a plasmid for restriction-ligation subcloning of DNA encoding amino acids 414 to 472 of the cytosolic tail of the human CB1 receptor (CB1414- 442).
  • Description: E. coli strain for the production of plasmid DNA carrying kanamycin resistance and containing an insert with the coding sequence for the polypeptide spanning amino acids 414 to 442 of the cytosolic tail of the human CB1 receptor (CB1414– 442) with BamHI and NotI restriction sites for subcloning.
  • Research area: Neuroscience, Cell Biology, Molecular Biology, Biochemistry
  • Animal free: Gómez-Caballero et al. 2021. Mikrochim Acta. 188(11):368. PMID: 34618242.

  • For Research Use Only

Target Details

Application Details

  • Application: Production of a plasmid for restriction-ligation subcloning of DNA encoding amino acids 414 to 472 of the cytosolic tail of the human CB1 receptor (CB1414- 442).

Handling

  • Format: Stock Glycerol
  • Storage conditions: -80 oC
  • Shipping conditions: Frozen in dry ice

Documentation

  • Available on request

References

  •   Transformed One Shot™ TOP10 E. coli