- Name Ann-Marie Broome
- Institute Medical University Of South Carolina
- Tool name: Biotinylated bioluminescent probe (YuLu)
- Alternate names: YuLu
- Tool type: Small molecules
- Tool sub-type: Marker
- Purpose: Marker
- Description: The global preclinical imaging market is estimated to reach 910 million in the US alone by 2021, including utilizing bioluminescent probes to study biological processes in vitro and in vivo in real time, image cancer cells, monitor gene delivery and adoptively transferred cells and track pathogen clearance, detect apoptosis and research signal transduction. Current limitations with many bioluminescence probes are the need for an external light source to excite the probe. Lucerifase based probes overcome this issue, however, D-luciferin or other small molecule substrates only last a very short time (15-20 minutes). This requires multiple applications for ongoing imaging, and would not be practical for applications such as tumor removal. There remains a need for a novel bioluminescent luciferase based probe, which does not rely on external light sources and is long lasting. MUSC researchers have synthesized a novel luciferase-based biotin containing bioluminescent probe, B-YL. This probe contains an aminoluciferin unit as the reporter, a PEG-1000 link for improving cell penetration and a biotin tail for binding to streptavidin. For proof-of-concept, the probe was fitted with an EGF peptide that binds to the EGF receptor, which is overexpressed in high-grade gliomas. In vivo bioluminescence signals of xenograft U87-luc brain tumors was done using the EGF-B-SA-B-YL probe and compared with an untargeted B-SA-B-YL probe. Maximum bioluminescence for the probe was recorded at 24 hours and lasted for six days. In addition, the probe targets are numerous and can be easily modified because of the SA and biotin system.
- Research area: Fluorescent Cell Imaging; Other
- For Research Use Only
- • Jiang et al. 2018. ACS Chem Neurosci. 9(1):100-106. PMID: 28532151.
- • Biotinylated Bioluminescent Probe for Long Lasting Targeted in Vivo Imaging of Xenografted Brain Tumors in Mice.