Water-soluble monovalent copper ligand-2 (MCL-2)
- Name Christoph J. Fahrni
- Institute Georgia Institute Of Technology
Tool name: Water-soluble monovalent copper ligand-2 (MCL-2)
Alternate names: MCL-2
Tool type: Small molecules
Tool sub-type: Inhibitor
Application: determination of Cu(I) binding affinities of proteins and small-molecule ligands
Description: Copper is an essential trace element that is central to a broad range of biological processes, including cellular respiration, connective tissue formation, pigment synthesis, antioxidant defense, and photosynthesis in plants and bacteria. The measurement of reliable Cu(I) protein binding affinities requires competing reference ligands with similar binding strengths; however, the literature on such reference ligands is not only sparse but often conflicting. To address this deficiency, Dr. Fahrni has created and characterized a series of water-soluble monovalent copper ligands, MCL-1, MCL-2, and MCL-3, that form well-defined, air-stable, and colorless complexes with Cu(I) in aqueous solution. X-ray structural data, electrochemical measurements, and an extensive network of equilibrium titrations showed that all three ligands form discrete Cu(I) complexes with 1:1 stoichiometry and are capable of buffering Cu(I) concentrations between 10â10 and 10â17 M.
Research area: Drug Discovery & Development; Other
- For Research Use Only
- • Bagchi et al. 2013. J Am Chem Soc. 135(49):18549-59. PMID: 24298878.
- • Robust affinity standards for Cu(I) biochemistry.