Product Image

Contributor Information

  • Name Christoph J. Fahrni
  • Institute Georgia Institute Of Technology

Tool Details

  • Tool name: Water-soluble monovalent copper ligand-2 (MCL-2)
  • Alternate names: MCL-2
  • Tool type: Small molecules
  • Tool sub-type: Inhibitor
  • Application: determination of Cu(I) binding affinities of proteins and small-molecule ligands
  • Description: Copper is an essential trace element that is central to a broad range of biological processes, including cellular respiration, connective tissue formation, pigment synthesis, antioxidant defense, and photosynthesis in plants and bacteria. The measurement of reliable Cu(I) protein binding affinities requires competing reference ligands with similar binding strengths; however, the literature on such reference ligands is not only sparse but often conflicting. To address this deficiency, Dr. Fahrni has created and characterized a series of water-soluble monovalent copper ligands, MCL-1, MCL-2, and MCL-3, that form well-defined, air-stable, and colorless complexes with Cu(I) in aqueous solution. X-ray structural data, electrochemical measurements, and an extensive network of equilibrium titrations showed that all three ligands form discrete Cu(I) complexes with 1:1 stoichiometry and are capable of buffering Cu(I) concentrations between 10−10 and 10−17 M.
  • Research area: Drug Discovery & Development; Other

  • For Research Use Only

Target Details

  • Primary target: Copper I; Cu(I)

Application Details

  • Application: determination of Cu(I) binding affinities of proteins and small-molecule ligands

Handling

  • Solubility: water

Documentation

Related Tools

Product Image

cis-SGK3-PROTAC1 small molecule (tool compound)

#158379
Product Image

Fungi Endoplasmic Reticulum Tracker 6 small molecule (tool compound)

#157734
Product Image

BioGraphene Gr-RS

#153983

References

  • •  Bagchi et al. 2013. J Am Chem Soc. 135(49):18549-59. PMID: 24298878.
  • •  Robust affinity standards for Cu(I) biochemistry.