Fluorescent probe for Copper
- Name Christoph J. Fahrni
- Institute Georgia Institute Of Technology
Tool name: Fluorescent probe for Copper
Tool type: Small molecules
Tool sub-type: Fluorescent Probe
Application: determination of Cu(I) binding affinities of proteins and small-molecule ligands
Description: Copper is an essential trace element that is central to a broad range of biological processes, including cellular respiration, connective tissue formation, pigment synthesis, antioxidant defense, and photosynthesis in plants and bacteria. The measurement of reliable Cu(I) protein binding affinities requires competing reference ligands with similar binding strengths; however, the literature on such reference ligands is not only sparse but often conflicting. Here, Dr. Farhrni has designed a water-solube fluorescence probe for the detection of aqueous Cu(I). This probe has a high selectivity (57-fold) for Cu(I) compared to other cations such as Cu(II), Hg(II), and Cd(II).
Research area: Drug Discovery & Development; Other
- For Research Use Only
- • Morgan et al. 2013. Dalton Trans. 42(9):3240-8. PMID: 23169532.
- • High-contrast fluorescence sensing of aqueous Cu(I) with triarylpyrazoline probes: dissecting the roles of ligand donor strength and excited state proton transfer.