Fungi Endoplasmic Reticulum Tracker 2 small molecule (tool compound)
- Name Micha Fridman
- Institute Tel Aviv University
Tool name: Fungi Endoplasmic Reticulum Tracker 2 small molecule (tool compound)
Tool type: Small molecules
Tool sub-type: Fluorescent Probe
Application: Tracking of the ER in live fungal cells (e.g. Candida).
Description: In fungal cells, the endoplasmic reticulum (ER) harbours several of the enzymes involved in the biosynthesis of ergosterol, an essential membrane component, making this organelle the site of action of antifungal azole drugs, used as a first-line treatment for fungal infections. This marker provides specific fluorescent labelling of this organelle in cells of live pathogenic fungi. Specifically, the marker is an antifungal azole-based fluorescent tracking reagent of the ER in live fungal cells.The fungal ER tracker is a unique molecular tool useful in the study of the ER and its interactions with other organelles in live cells of pathogenic fungi.
Research area: Cell Type or Organelle Marker; Cell Structure and Motility; Fluorescent Cell Imaging
Selectivity: Highly specific for Endoplasmic Reticulum.
Additional notes: This tracker is optimised for use in fungi, not mammalian or plant cells and has shown superiority to other commercially available ER trackers. It has been evaluated in a panel of Candida, including C. albicans and C. glabrata which are considered the two most common fungal pathogens relevant to human infection. There is weak background signal and high specificity.
- For Research Use Only
- • Benhamou et al. 2018. ACS Chem Biol. 13(12):3325-3332. PMID: 30427174.