Product Image

Contributor Information

  • Name Jiri Strohalm ; Karel Ulbich ; Petr Cigler ; Pavel Majer ; Frantisek Sedlak ; Tomas Knedlik ; Jirí Schimer ; Jan Konvalinka ; Pavel Sacha ; Sub Vladlmir ; Jan Tykvart ; Vaclav Navratil
  • Institute Institute of Organic Chemistry and Biochemistry ; Charles University ; Institute of Macromolecular Chemistry

Tool Details

  • Tool name: Negative control iBody (ATTO488)
  • Tool type: Small molecules
  • Tool sub-type: Marker
  • Purpose: Marker
  • Description: - iBodies™ are capable of replacing antibodies in biomedical applications such as ELISA, flow cytometry, confocal microscopy, immunocytochemistry, Western Blot and immunoprecipitation. - These iBodies™ consist of an N-(2-hydroxypropyl)methacrylamide (HPMA) copolymer decorated with low-molecular-weight compounds that function as targeting ligand (e.g., enzyme inhibitors, receptor ligands), affinity anchor (e.g., biotin) and/or imaging probe (various fluorophores). - These iBodies™ have been used for enzyme inhibition, protein isolation, immobilization, quantification and live cell imaging as well as for classical Western Blot application. The system is remarkably modular and can be used for different biological/biochemistry assays.
  • Research area: Cancer

  • For Research Use Only

Target Details

Application Details

Handling

  • Storage conditions: Storage at -20°C; solubility H2O; stable at RT, 4°C and -80°C
  • Shipping conditions: Dry Ice

Documentation

  • Available on request

References

  •   Ĺ imon et al. 2018. ACS Chem Biol. 13(12):3333-3342. PMID: 30489064.
  •   DvořákovĂĄ et al. 2017. J Med Chem. 60(20):8385-8393. PMID: 28953383.
  •   Ĺ ĂĄcha et al. 2016. Angew Chem Int Ed Engl. 55(7):2356-60. PMID: 26749427.