SMALL MOLECULES
Contributor Information
- Name Jiri Strohalm ; Karel Ulbich ; Petr Cigler ; Pavel Majer ; Frantisek Sedlak ; Tomas Knedlik ; Jirí Schimer ; Jan Konvalinka ; Pavel Sacha ; Sub Vladlmir ; Jan Tykvart ; Vaclav Navratil
- Institute Institute of Organic Chemistry and Biochemistry ; Charles University ; Institute of Macromolecular Chemistry
Tool Details
- Tool name: Negative control iBody (ATTO488)
- Tool type: Small molecules
- Tool sub-type: Marker
- Purpose: Marker
- Description: - iBodies are capable of replacing antibodies in biomedical applications such as ELISA, flow cytometry, confocal microscopy, immunocytochemistry, Western Blot and immunoprecipitation. - These iBodies consist of an N-(2-hydroxypropyl)methacrylamide (HPMA) copolymer decorated with low-molecular-weight compounds that function as targeting ligand (e.g., enzyme inhibitors, receptor ligands), affinity anchor (e.g., biotin) and/or imaging probe (various fluorophores). - These iBodies have been used for enzyme inhibition, protein isolation, immobilization, quantification and live cell imaging as well as for classical Western Blot application. The system is remarkably modular and can be used for different biological/biochemistry assays.
- Research area: Cancer
- Additional notes: HPMA conjugate characteristics: MW: 78 041 g/mol #Biotins/molecule: 8 Fluorochrome name: ATTO488 #Fluorochrome/molecule: 5
- For Research Use Only
Target Details
Application Details
Handling
- Storage conditions: Storage at -20°C; solubility H2O; stable at RT, 4°C and -80°C
- Shipping conditions: Dry Ice
Documentation
- Available on request
Related Tools
References
- • Ĺ imon et al. 2018. ACS Chem Biol. 13(12):3333-3342. PMID: 30489064.
- • DvoĹĂĄkovĂĄ et al. 2017. J Med Chem. 60(20):8385-8393. PMID: 28953383.
- • Ĺ ĂĄcha et al. 2016. Angew Chem Int Ed Engl. 55(7):2356-60. PMID: 26749427.
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