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Contributor Information

  • Name Lloyd Murfin ; Tony James ; Simon Lewis
  • Institute University of Bath

Tool Details

  • Tool name: AzuFluorTM 483-Bpin: Azulene-Based Fluorescent Probe for ROS/RNS small molecule (tool compound)
  • Alternate names: AzuFluorTM 483-Bpin, Diethyl 2-amino-6-(4,4,5,5-tetramethyl-1,3,2-dioxaborolan-2-yl)azulene-1,3- dicarboxylate
  • Molecular formula: C22H28BNO6
  • Tool type: Small molecules
  • Tool sub-type: Fluorescent Probe
  • Molecular weight of the target: 412.2046
  • Description: Reactive oxygen species (ROS) and reactive nitrogen species (RNS) are important mediators in many physiological and pathological processes. The one-electron reduction of O2 in vivo leads to the formation of O2- (superoxide), which in turn undergoes disproportionation catalyzed by superoxide dismutase to give O2 and H2O2 (hydrogen peroxide). Another fate of O2- is to react with endogenously produced NO (nitric oxide) to form ONOO- (peroxynitrite) via a nonenzymatic process. Ordinarily, the flux of H2O2 is tightly regulated; aberrant H2O2 production or overexposure is implicated in the pathogenesis of many diseases such as cancer and neurodegenerative conditions. Similarly, while ONOO- has roles in signal transduction, its strongly oxidizing and nitrating properties mean it can react in an uncontrolled manner with various biomolecules. Elevated levels of ONOO- have been linked to cardiovascular, neurodegenerative, and inflammatory diseases as well as cancer. In view of this, there is significant need for tools and techniques to elucidate the roles of ONOO-, H2O2, and other ROS/RNS in biological systems.
  • Research area: Cancer
  • Additional notes: pH: Fluorescence intensity greatest at pH 7-8. Optimal two-photon excitation wavelength Îťex = 800nm.

  • For Research Use Only

Target Details

  • Target molecular weight: 412.2046
  • Primary target: Reactive Oxygen and Nitrogen Species

Application Details


  • Purity: 412.2046 g/mol
  • Solubility: DMSO, MeCN, Acetone, MeOH
  • Storage conditions: Ambient
  • Shipping conditions: Dry Ice


  • Available on request


  •   Murfin et al. 2019. J Am Chem Soc. 141(49):19389-19396. PMID: 31773957.